pECE-HA-AMPKalpha2 wt质粒载体BioVector NTCC保藏中心

pECE-HA-AMPKalpha2 wt,

BioVector Plasmid 031654.

Gene/Insert:protein kinase, AMP-activated, alpha 2 catalytic subunit (Homo sapiens)

Vector backbone pECE, plasmid 26453

Backbone size w/o insert (bp)2900

Vector typeMammalian Expression

GROWTH IN BACTERIA

Bacterial Resistance(s)Ampicillin

Growth Temperature37°C

Growth Strain(s)DH5alpha

Copy numberHigh Copy

GENE/INSERT

Gene/Insert nameprotein kinase, AMP-activated, alpha 2 catalytic subunit

Alt namePRKAA2

SpeciesH. sapiens (human)

Insert Size (bp)1660

Entrez GenePRKAA2 (a.k.a. AMPK, AMPK2, AMPKa2, PRKAA)

Tag / Fusion Protein

HA (YDVPDYASL) (N terminal on backbone)

CLONING INFORMATION

Cloning methodRestriction Enzyme

5′ cloning siteEcoRI (not destroyed)

3′ cloning siteXbaI (not destroyed)

5′ sequencing primerSV40pro-F

3′ sequencing primerSV40pA-R