pMJ806原核基因编辑质粒CRISPR载体-BioVector NTCC保藏中心

pMJ806,

Plasmid BioVector-039312.

Vector backbone pEC-K-MBP

Backbone manufacturerpET-based custom vector

Backbone size w/o insert (bp)6400

Total vector size (bp)10500

Vector typeBacterial Expression, CRISPR

GROWTH IN BACTERIA

Bacterial Resistance(s)Kan 25ug/mL

Growth Temperature37°C

Growth Strain(s)XL1 Blue

Growth instructions Low Kan concentration required, use 25ug/mL.

Copy number Low Copy

GENE/INSERT

Gene/Insert nameCas9

Alt nameCsn1

SpeciesStreptococcus pyogenes

Insert Size (bp)4104

GenBank IDAAK33936.1 13622193

Promoter T7

Tags / Fusion Proteins

MBP (N terminal on backbone)

His6 (N terminal on backbone)

CLONING INFORMATION

Cloning methodLigation Independent Cloning

5′ sequencing primerMBP-forward

3′ sequencing primerT7 terminator