p426-SNR52p-gRNA.CAN1.Y-SUP4t plasmid酵母基因编辑质粒载体-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

p426-SNR52p-gRNA.CAN1.Y-SUP4t

Yeast Expression, CRISPRChurchCAN1.y gRNA (Saccharomyces cerevisiae)Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res

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PURPOSE

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Encodes a gRNA that targets Can1.Y in yeast.

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gRNA target sequence GATACGTTCTCTATGGAGGA

BACKBONE

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Vector backbone

· p426

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Backbone size w/o insert (bp)5886

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Total vector size (bp)6274

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Vector type

· Yeast Expression, CRISPR

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Selectable markers

· URA3

GROWTH IN BACTERIA

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Bacterial Resistance(s)

· Ampicillin

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Growth Temperature

· 30°C

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Growth Strain(s)

· DH5alpha

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Copy number

· High Copy

GENE/INSERT

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Gene/Insert name

· CAN1.y gRNA

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Species

· S. cerevisiae (budding yeast)

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Insert Size (bp)

· 488

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Entrez Gene

· CAN1(a.k.a. YEL063C)

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PromoterSNR52

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CLONING INFORMATION

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Cloning methodUnknown

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5′ sequencing primerT3 promoter

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3′ sequencing primerT7 promoter

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