LL 97a cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

LL 97a cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Organism Homo sapiens, human
Tissue lung
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent

Karyotype diploid; relatively unstable
Clinical Data
48 years
Caucasian
male
Complete Growth Medium Ham's F12K medium, 85%; fetal bovine serum, 15%
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C

STR Profile
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,13
D16S539: 11,13
D5S818: 13
D7S820: 9,11
THO1: 6,9.3
TPOX: 10,11
vWA: 14,17
Isoenzymes
G6PD, B
【Supplier来源】BioVector NTCC Inc.
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【Website网址】 http://www.biovector.net