AAV.PHP腺相关病毒衣壳包装质粒四环素调控表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥49865
- 货 号：AAV.PHP腺相关病毒衣壳包装质粒四环素调控表达载体
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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AAV.PHP腺相关病毒衣壳包装质粒四环素调控表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold. This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells.
The PHP.S serotype exhibits efficient transduction of the peripheral nervous system via systemic delivery in adult animals. In vivo studies showed that, relative to parent capsid AAV9, intravenous injection of PHP.S AAV led to an increase in both the number of transduced cells and the expression level per cell. PHP.S AAV was also shown to transduce neurons in the enteric nervous system and in other peripheral ganglia, such as the cardiac ganglia. When used with Cre transgenic lines or cell-type-specific promoters, this serotype could enable targeted transduction of subsets of cells within these regions.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold. This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells.
The PHP.S serotype exhibits efficient transduction of the peripheral nervous system via systemic delivery in adult animals. In vivo studies showed that, relative to parent capsid AAV9, intravenous injection of PHP.S AAV led to an increase in both the number of transduced cells and the expression level per cell. PHP.S AAV was also shown to transduce neurons in the enteric nervous system and in other peripheral ganglia, such as the cardiac ganglia. When used with Cre transgenic lines or cell-type-specific promoters, this serotype could enable targeted transduction of subsets of cells within these regions.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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