pJAK-109产黄青霉红色荧光表达载体质粒Penicillium chrysogenum expression plasmid BioVector NTCC质粒载体菌种细胞基因保藏中心

pJAK-109产黄青霉红色荧光表达载体质粒Penicillium chrysogenum expression plasmid BioVector NTCC质粒载体菌种细胞基因保藏中心
Maintenance of the DsRed-SKL expression plasmid pJAK-109 in P.
chrysogenum grown on different media. Upon selection on acetamide medium,
all colonies show expression of DsRed-SKL (dark-grey colonies). Harvested
spores were plated on R-Agar, and plasmid loss occurred without selection
pressure in approximately half of the colonies (light-grey colonies vs. dark-grey
colonies). Further plating of spores from colonies without red fluorescence did
not yield colonies on acetamide medium. Plating of spores from colonies
showing red fluorescence resulted in a high number of colonies without the
DsRed-SKL expression. b) DsRed-SKL fluorescence of P. chrysogenum with
the pJAK-109 plasmid, grown on acetamide or R-agar medium.
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