PPARγ-LBD表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

PPARγ-LBD表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
Peroxisome Proliferator Activated Receptor gamma Ligand Binding Domain
 Species: Human
 Molecular Weight: 33.6 kDa.
 Gene Accession Number: NM_138712.
Background
There is evidence that a group of closely related nuclear receptors, called peroxisome proliferator-activated receptors (PPARs), may be
involved in chronic diseases such as diabetes, obesity, artherosclerosis and cancer. The PPARs were first cloned as the nuclear receptors
that mediate the effects of synthetic compounds called peroxisome proliferators on gene transcription. It soon became clear that eicosanoids
and fatty acids can also regulate gene transcription through PPARs. They bind a specific element in the promoter region of target genes only
as a heterodimer with the receptor for 9- cis retinoic acid, RXR (retinoid X receptor). Binding of the ligand of either receptor can activate the
complex, but binding of both ligands simultaneously is more potent (1). Three PPAR isotypes have been identified: α, β (also called NUC1)
and γ. PPARα is expressed most in brown adipose tissue and liver, then kidney, heart and skeletal muscle. PPARγ is mainly expressed in
adipose tissue, and to a lesser extent in colon, the immune system and the retina. PPARβ is found in many tissues but the highest
expression is in the gut, kidney and heart (2). PPARγ influences the storage of fatty acids in the adipose tissue. With the C/EBP transcription
factors, PPARγ is part of the adipocyte differentiation program that induces the maturation of pre-adipocytes into fat cells. Most of the PPARγ
target genes in adipose tissue are directly implicated in lipogenic pathways, including lipoprotein lipase (LPL), adipocyte fatty acid binding
protein (A-FABP or AP2), acyl-CoA synthase and fatty acid transport protein (FATP) (3). In addition, PPARγ is a direct target gene of the
transcription factor sterol response element binding protein 1 (SREBP1) emphasizing the cooperative and additive functions between these
two types of receptor (4).
Applications
PPARγ can be applied in DNA and protein-protein interactions assays.
Protein Sequence
LNPESADLRA LAKHLYDSYI KSFPLTKAKA RAILTGKTTD KSPFVIYDMN SLMMGEDKIK
FKHITPLQEQ SKEVAIRIFQ GCQFRSVEAV QEITEYAKSI PGFVNLDLND QVTLLKYGVH
EIIYTMLASL MNKDGVLISE GQGFMTREFL KSLRKPFGDF MEPKFEFAVK FNALELDDSD
LAIFIAVIIL SGDRPGLLNV KPIEDIQDNL LQALELQLKL NHPESSQLFA KLLQKMTDLR
QIVTEHVQLL QVIKKTETDM SLHPLLQEIY KDLY
References
1. Desvergene et al., (1999) Endocr. Rev. 20, 649-688
2. Kersten (2000) Nature 405, 421-424
3. Rosen et al., (1999) Mol. Cell 4, 611-617
4. Fajas et al., (1999) Mol. Cell. Biol. 19, 5495-550
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