pAWP89 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pAWP89 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
Methylomonas sp. LW13 Type I methanotroph, wild type 13
Methylomonas sp. LW13-glgA::km Methylomonas sp. LW13 glgA::Kmr This work
M. tundripaludum 21/22 Type I methanotroph, wild type 13
M. tundripaludum 21/22-glgA2::km M. tundripaludum strain 21/22 glgA2::Kmr This work
M. buryatense
5GB1 Variant of M. buryatense 5G 9
5GB1C Variant of M. buryatense 5GB1 intentionally cured of its native plasmid and capable
of being conjugated with small IncP-based plasmids
9
5GB1C-glgA1::km M. buryatense 5GB1C glgA1::Kmr This work
5GB1C-glgA1 M. buryatense 5GB1C glgA1 This work
5GB1C-mmoD::km M. buryatense 5GB1C mmoD::Kmr This work
5GB1C-mmoD M. buryatense 5GB1C mmoD This work
5GB1C-smmo::km M. buryatense 5GB1C sMMO::Kmr This work
5GB1C-smmo M. buryatense 5GB1C sMMO This work
5GB1C-Ppmo-xylE M. buryatense 5GB1C with Ppmo-xylE inserted between the METBUDRAFT_2794
and METBUDRAFT_2795 genes
This work
Plasmids
pAWP89 An IncP-based broad-host-range plasmid containing dTomato driven by the tac
promoter, Kmr
9
pCM433kanT A broad-host-range sacB-based vector for unmarked allelic exchange, Kmr 9
pCM433-moD pCM433kanT containing the deletion construct of mmoD This work
pET-29b( ) Expression vector, Kmr Novagen
pET29-M pET-29b( ) containing the METBUDRAFT_0046 gene, Kmr This work
pFC25 A broad-host-range plasmid containing flp driven by the tac promoter, Hmr This work
P7Z6 pMD18-T containing the zeocin resistance marker, Zeor 18
pCM158 A broad-host-range cloning vector, Kmr 20
pTEC27 Template for the hygromycin resistance marker, Hmr Addgene (21)
pE-FLP A temperature-sensitive plasmid containing the flp gene, Ampr Addgene (22)
pCM130 xylE promoter-probe vector, Tetr
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