JA221 E.coli大肠杆菌菌株感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

JA221 E.coli大肠杆菌菌株感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心
Name:
Escherichia coli (Migula 1895) Castellani and Chalmers 1919
Strain designation:
JA221
Date of sampling:
before 26.01.1989
Cultivation conditions:
LB (Luria-Bertani) MEDIUM, 37°C

LB (Luria-Bertani) MEDIUM
Tryptone 10.0 g
Yeast extract 5.0 g
NaCl 10.0 g
Agar 20.0 g
Distilled water 1000.0 ml
Adjust pH to 7.0.
Risk group:
1

Cultivation conditions:
LB (Luria-Bertani) MEDIUM, 37°C

LB (Luria-Bertani) MEDIUM
Tryptone 10.0 g
Yeast extract 5.0 g
NaCl 10.0 g
Agar 20.0 g
Distilled water 1000.0 ml
Adjust pH to 7.0.

• Sex(Hfr,F+,F-,or F'): F-
• 16 Mutations:
Mutation Location Certainty Display
araC14 1.52 1 araC14
leuB6(Am) 1.74 1 leuB6(Am)
lacY1 7.79 1 lacY1
glnX44(AS) 14.99 1 glnX44(AS)
galK2(Oc) 16.99 1 galK2(Oc)
galT22 17.01 1 galT22
LAM- 17.40 1 λ-
trp-5 28.33 1 trp-5
rfbC1 45.43 0 rfbC1
mgl-51 48.17 0 mgl-51
recA1 60.80 1 recA1
xylA5 80.34 1 xylA5
mtl-1 81.26 1 mtl-1
thiE1 90.34 1 thiE1
hsdR4 98.74 1 hsdR4
ValR 1000.00 1 ValR
•
• Comments:
• References:
o Chinault, A.C., J. Carbon 1979. Overlap hybridization screening: isolation and characterization of overlapping DNA fragments surrounding the leu2 gene on yeast chromosome III. Gene 5:111-126

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