pNRW75 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pNRW75 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
Serratia sp. LIS Serratia ATCC 39006 smaI : : miniTn5Sm/Sp, SpR Thomson et al. (2000) S. marcescens ATCC 274 (Sma 274) Wild-type (Pig+), non-motile Lab stock S. marcescens strain 12 (Sma 12) Wild-type (Pig2), clinical isolate Harris et al. (2004) S. marcescens ATCC 274 motile isolate (Sma M274) Wild-type (Pig+), motile Matsuyama et al. (1995) NW7 Sma 274, copA : :cat, CmR Williamson et al. (2006) SCC4 Sma 274, luxS : : KmR , KmR Coulthurst et al. (2004) Sma 12I smaI : : miniTn5km1, KmR This study Sma 12P pig+, copA : :cat, CmR derivative of Sma 12; generated by transduction using w3M grown on strain NW7 This study Sma 12IP pig+, smaI : : miniTn5km1, CmR KmR derivative of Sma 12P; generated by transduction using w3M grown on strain Sma 12I This study Sma 12S luxS : : KmR , KmR derivative of Sma 12; generated by transduction using w3M grown on strain SCC4 This study Sma 12SP luxS : : KmR , pig+, copA : :cat, CmR KmR derivative of Sma 12S; generated by transduction using w3M grown on strain NW7 This study Sma 274I smaI : : miniTn5km1, smaR+ derivative of Sma 274 following transduction using w3M grown on strain Sma 12I This study Sma M274I smaI : : miniTn5km1, smaR+ derivative of Sma M274 following transduction using w3M grown on strain Sma 12I This study Other bacterial strains Chromobacterium violaceum CV026 cviI : : Tn5, aHSL bioassay strain McClean et al. (1997) Vibrio harveyi BB170 luxN : : Tn5, AI-2 bioassay strain Surette et al. (1999) Phage w3M S. marcescens generalized transducing phage Williamson et al. (2006) Plasmids pACYC184 Low-copy cloning vector, CmR , TcR Chang & Cohen (1978) pACYC177 Low-copy cloning vector, KmR , ApR Chang & Cohen (1978) pNRW75 675 bp PCR-amplified fragment containing smaI from Sma 12 in the EcoRI site of pACYC184 This study pNRW85 889 bp PCR-amplified fragment containing smaR from Sma 12 in the PstI site of pACYC177 This study pUTminiTn5Km1 Delivery plasmid for miniTn5Km1, ApR , KmR de Lorenzo et al. (1990)
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