DH10B strain菌株菌种感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

DH10B strain菌株菌种感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心
DH10B (Invitrogen)
str. K-12 F– Δ(ara-leu)7697[Δ(rapA'-cra' )] Δ(lac)X74[Δ('yahH-mhpE)] duplication(514341-627601)[nmpC-gltI] galK16 galE15 e14–(icdWT mcrA) φ80dlacZΔM15 recA1 relA1 endA1 Tn10.10 nupG rpsL150(StrR) rph+ spoT1 Δ(mrr-hsdRMS-mcrBC) λ– Missense(dnaA glmS glyQ lpxK mreC murA) Nonsense(chiA gatZ fhuA? yigA ygcG) Frameshift(flhC mglA fruB)

An MC1061 derivative (Casadaban80). Prepare cells for chemical transformation with CCMB80 buffer
Probably identical to Thermo TOP10 and NEB 10-beta commercial strains.
Recombination-deficient (recA1)
Endonuclease I-deficient (endA1)
Relaxed phenotype (relA1)
Constitutive deoxyribose synthesis for improving cloning of large plasmids
Suitable for cloning methylated cytosine or adenine containing DNA
High competence (galE–)
Blue/white screening with lacZα.
While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B (and by their lineage also TOP10 and any other MC1061 derivatives) is actually (galE galK galU)+. Dcekiert 16:37, 23 January 2008 (CST)
Genome sequence indicates that DH10B is actually deoR+. Presumably TOP10 and MC1061 are also deoR+.
Streptomycin-resistant and 2-deoxygalactose-resistant
Leucine auxotroph
Arabinose, lactose, and galactose non-utilizing
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