ALL-SIL急性淋巴细胞B细胞白血病细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

ALL-SIL急性淋巴细胞B细胞白血病细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line: ALL-SIL DSMZ no.: ACC 511Species: human (Homo sapiens)Cell type: T cell leukemia Origin: established from the peripheral blood of a 17-year-old man with T-ALL (T cell acute lymphoblastic leukemia) at relapse; also found in the literature as SIL-ALL; carries NUP214-ABL1 fusion gene Reference(s): 14489, 14574, 15217Biosafety level: 1 Permissions and restrictions: A DSMZ Cell Culture Data: Morphology:mostly round cells growing singly or in small clusters in suspension; image; imageMedium: 80-90% RPMI 1640 + 10-20% h.i. FBS Subculture: seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml; maximal density of ca. 1.5 x 106 cells/ml; it may be of advantange to first culture the cells after thawing in a 24-well-plate with 20% FBS Incubation: at 37 °C with 5% CO2 Doubling time: ca. 48 hours Harvest: cell harvest of about 1.5 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO DSMZ Scientific Data: Mycoplasma: contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, RNA hybridization, PCR assays Immunology: cyCD3 +, CD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile Species: confirmed as human by cytogenetics and DNA fingerprint Cytogenetics: human hypertetraploid karyotype - 90-95<4n>XX/XXYY, +6, +8, +8, t(1;13)(p32;q32)x2, del(6)(q25)x2, del(9)(?p23p24)x2, t(10;14)(q24;q11.2)x2, add(17)(p11)x2 - sideline with idem, -20, -20 - carries t(10;14) effecting TLX1-TRD (HOX11-TCRD) juxtaposition - matches published karyotype Molec. Genetics: expression of TLX1 confirmed by RT-PCR Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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