- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pLSLR plasmid
Single component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.
Backbone size (bp)10531
- Modifications to backbonecis-acting replicational elements from the bean yellow dwarf virus were added in an LIR-SIR-Rep/RepA-LIR orientation.
- Vector typeplant T-DNA plasmid
- Selectable markersHygromycin
- Bacterial Resistance(s)Kanamycin
- Growth Temperature37°C
- Growth Strain(s)DH5alpha
- Copy numberHigh Copy
Cloning methodRestriction Enzyme
5′ sequencing primertcccactgacttgaagtacac
3′ sequencing primerggacttgtttagagtttcta
Map:
Sequence序列:
>pLSLR sequence 10531 bps
caaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacctggtggagaagttgaaggccgcg
caggccgcccagcggcaacgcatcgaggcagaagcacgccccggtgaatcgtggcaagcggccgctgatc
gaatccgcaaagaatcccggcaaccgccggcagccggtgcgccgtcgattaggaagccgcccaagggcga
cgagcaaccagattttttcgttccgatgctctatgacgtgggcacccgcgatagtcgcagcatcatggac
gtggccgttttccgtctgtcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccagacg
ggcacgtagaggtttccgcagggccggccggcatggccagtgtgtgggattacgacctggtactgatggc
ggtttcccatctaaccgaatccatgaaccgataccgggaagggaagggagacaagcccggccgcgtgttc
cgtccacacgttgcggacgtactcaagttctgccggcgagccgatggcggaaagcagaaagacgacctgg
tagaaacctgcattcggttaaacaccacgcacgttgccatgcagcgtacgaagaaggccaagaacggccg
cctggtgacggtatccgagggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccgggcgg
ccggagtacatcgagatcgagctagctgattggatgtaccgcgagatcacagaaggcaagaacccggacg
tgctgacggttcaccccgattactttttgatcgatcccggcatcggccgttttctctaccgcctggcacg
ccgcgccgcaggcaaggcagaagccagatggttgttcaagacgatctacgaacgcagtggcagcgccgga
gagttcaagaagttctgtttcaccgtgcgcaagctgatcgggtcaaatgacctgccggagtacgatttga
aggaggaggcggggcaggctggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagcatc
cgccggttcctaatgtacggagcagatgctagggcaaattgccctagcaggggaaaaaggtcgaaaaggc
ctctttcctgtggatagcacgtacattgggaacccaaagccgtacattgggaaccggaacccgtacattg
ggaacccaaagccgtacattgggaaccggtcacacatgtaagtgactgatataaaagagaaaaaaggcga
tttttccgcctaaaactctttaaaacttattaaaactcttaaaacccgcctggcctgtgcataactgtct
ggccagcgcacagccgaagagctgcaaaaagcgcctacccttcggtcgctgcgctccctacgccccgccg
cttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcctacggccaggcaatctaccagg
gcgcggacaagccgcgccgtcgccactcgaccgccggcgcccacatcaaggcaccctgcctcgcgcgttt
cggtgatgacggtgaaaacctctgacacatgcagctcccggaaacggtcacagcttgtctgtaagcggat
gccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgaccc
agtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagtg
caccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgctt
cctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggt
aatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggcc
aggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaa
atcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaag
ctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggga
agcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgg
gctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaa
cccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgta
ggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatct
gcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgc
tggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcct
ttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgcatt
ctaggtactaaaacaattcatccagtaaaatataatattttattttctcccaatcaggcttgatccccag
taagtcaaaaaatagctcgacatactgttcttccccgatatcctccctgatcgaccggacgcagaaggca
atgtcataccacttgtccgccctgccgcttctcccaagatcaataaagccacttactttgccatctttca
caaagatgttgctgtctcccaggtcgccgtgggaaaagacaagttcctcttcgggcttttccgtctttaa
aaaatcatacagctcgcgcggatctttaaatggagtgtcttcttcccagttttcgcaatccacatcggcc
agatcgttattcagtaagtaatccaattcggctaagcggctgtctaagctattcgtatagggacaatccg
atatgtcgatggagtgaaagagcctgatgcactccgcatacagctcgataatcttttcagggctttgttc
atcttcatactcttccgagcaaaggacgccatcggcctcactcatgagcagattgctccagccatcatgc
cgttcaaagtgcaggacctttggaacaggcagctttccttccagccatagcatcatgtccttttcccgtt
ccacatcataggtggtccctttataccggctgtccgtcatttttaaatataggttttcattttctcccac
cagcttatataccttagcaggagacattccttccgtatcttttacgcagcggtatttttcgatcagtttt
ttcaattccggtgatattctcattttagccatttattatttccttcctcttttctacagtatttaaagat
accccaagaagctaattataacaagacgaactccaattcactgttccttgcattctaaaaccttaaatac
cagaaaacagctttttcaaagttgttttcaaagttggcgtataacatagtatcgacggagccgattttga
aaccgcggtgatcacaggcagcaacgctctgtcatcgttacaatcaacatgctaccctccgcgagatcat
ccgtgtttcaaacccggcagcttagttgccgttcttccgaatagcatcggtaacatgagcaaagtctgcc
gccttacaacggctctcccgctgacgccgtcccggactgatgggctgcctgtatcgagtggtgattttgt
gccgagctgccggtcggggagctgttggctggctggtggcaggatatattgtggtgtaaacaaattgacg
cttagacaacttaataacacattgcggacgtttttaatgtactgaattaacgccgaattaattcggggga
tctggattttagtactggattttggttttaggaattagaaattttattgatagaagtattttacaaatac
aaatacatactaagggtttcttatatgctcaacacatgagcgaaaccctataggaaccctaattccctta
tctgggaactactcacacattattatggagaaaatagagcttgtcgatcgttaattaatcacctccaaga
tcccggacgagtgctggggcgtcggtttccactatcggcgagtacttctacacagccatcggtccagacg
gccgcgcttctgcgggcgatttgtgtacgcccgacagtcccggctccggatcggacgattgcgtcgcatc
gaccctgcgcccaagctgcatcatcgaaattgccgtcaaccaagctctgatagagttggtcaagaccaat
gcggagcatatacgcccggagccgcggcgatcctgcaagctccggatgcctccgctcgaagtagcgcgtc
tgctgctccatacaagccaaccacggcctccagaagaagatgttggcgacctcgtattgggaatccccga
acatcgcctcgctccagtcaatgaccgctgttatgcggccattgtccgtcaggacattgttggagccgaa
atccgcgtgcacgaggtgccggacttcggggcagtcctcggcccaaagcatcagctcatcgagagcctgc
gcgacggacgcactgacggtgtcgtccatcacagtttgccagtgatacacatggggatcagcaatcgcgc
atatgaaatcacgccatgtagtgtattgaccgattccttgcggtccgaatgggccgaacccgctcgtctg
gctaagatcggccgcagcgatcgcatccatggcctccgcgaccggctgcagaacagcgggcagttcggtt
tcaggcaggtcttgcaacgtgacaccctgtgcacggcgggagatgcaataggtcaggctctcgctgaatt
ccccaatgtcaagcacttccggaatcgggagcgcggccgatgcaaagtgccgataaacataacgatcttt
gtagaaaccatcggcgcagctatttacccgcaggacatatccacgccctcctacatcgaagctgaaagca
cgagattcttcgccctccgagagctgcatcaggtcggacacgctgtcgaacttttcgatcagaaacttct
cgacagacgtcgcggtgagttcaggctttttcatcctaggagagatagatttgtagagagagactggtga
tttcagcgtgtcctctccaaatgaaatgaacttccttatatagaggaaggtcttgcgaaggatagtggga
ttgtgcgtcatcccttacgtcagtggagatatcacatcaatccacttgctttgaagacgtggttggaacg
tcttctttttccacgatgctcctcgtgggtgggggtccatctttgggaccactgtcggcagaggcatctt
gaacgatagcctttcctttatcgcaatgatggcatttgtaggtgccaccttccttttctactgtcctttt
gatgaagtgacagatagctgggcaatggaatccgaggaggtttcccgatattaccctttgttgaaaagtc
tcaatagccctttggtcttctgagactgtatctttgatattcttggagtagacgagagtgtcgtgctcca
ccatgttatcacatcaatccacttgctttgaagacgtggttggaacgtcttctttttccacgatgctcct
cgtgggtgggggtccatctttgggaccactgtcggcagaggcatcttgaacgatagcctttcctttatcg
caatgatggcatttgtaggtgccaccttccttttctactgtccttttgatgaagtgacagatagctgggc
aatggaatccgaggaggtttcccgatattaccctttgttgaaaagtctcaatagccctttggtcttctga
gactgtatctttgatattcttggagtagacgagagtgtcgtgctccaccatgtcgactatcggacgagct
caaagtttaacgcgttagcagaaggcatgttgttgtgactccgaggggttgcctcaaactctatcttata
accggcgtggaggcatggaggcaggggtattttggtcattttaatagatagtggaaaatgacgtggaatt
tacttaaagacgaagtctttgcgacaagggggggcccacgccgaatttaatattaccggcgtggcccccc
cttatcgcgagtgctttagcacgagcggtccagatttaaagtagaaaatttcccgcccactagggttaaa
ggtgttcacactataaaagcatatacgatgtgatggtatttgatggagcgtatattgtatcaggtatttc
cgttggatacgaattattcgtacgaccctcGGCGCGCCGAGAACTTCCAGAGCTTTGGGTACGTCACGTG
GCTCGAGCGCGTAGTCCTCGGTAATATCGCAGAACAAAAGTACCTGATATCgagtgtacttcaagtcagt
gggaaatcaataaaatgattattttatgaatatatttcattgtgcaagtagatagaaattacatatgtta
cataacacacgaaataaacaaaaaaagacaatccaaaaacaaacaccccaaaaaaaataatcactttaga
taaactcgtatgaggagaggcacgttcagtgactcgacgattcccgagcaaaaaaagtctccccgtcaca
catgtagtgggtgacgcaattatctttaaagtaatccttctgttgacttgtcattgataacatccagtct
tcgtcaggattgcaaagaattatagaagggatcccaccttttattttcttcttttttccatatttagggt
tgacagtgaaatcagactggcaacctattaattgcttccacaatgggacgaacttgaaggggatgtcgtc
gatgatattataggtggcgtgttcatcgtagttggtgaaatcgatggtaccgttccaatagttgtgtcgt
ccgagacttctagcccaggtggtctttccggtacgagttggtccgcagatgtagaggctggggtgtcgga
ttccattccttccattgtccttgttaaatcggccatccattcaaggtcagattgagcttgttggtatgag
acaggatgtatgtaagtataagcgtctatgcttacatggtatagatgggtttccctccaggagtgtagat
cttcgtggcagcgaagatctgattctgtgaagggcgacacatacggttcaggttgtggagggaataattt
gttggctgaatattccagccattgaagctttgttgcccattcatgagggaattcttccttgatcatgtca
agatattcctccttagacgttgcagtctggataatagttctccatcgtgcgtcagatttgcgaggagaaa
ccttatgatctcggaaatctcctctggttttaatatctccgtcctttgatatgtaatcaaggacttgttt
agagtttctagctggctggatattagggtgatttccttcaaaatcgaaaaaagaaggatccctaatacaa
ggttttttatcaagctggagaagagcatgatagtgggtagtgccatcttgatgaagctcagaagcaacac
caaggaagaaaataagaaaaggtgtgagtttctcccagagaaactggaataaatcatctctttgagatga
gcacttgggataggtaaggaaaacatatttagattggagtctgaagttcttactagcagaaggcatgttg
ttgtgactccgaggggttgcctcaaactctatcttataaccggcgtggaggcatggaggcaggggtattt
tggtcattttaatagatagtggaaaatgacgtggaatttacttaaagacgaagtctttgcgacaaggggg
ggcccacgccgaatttaatattaccggcgtggcccccccttatcgcgagtgctttagcacgagcggtcca
gatttaaagtagaaaatttcccgcccactagggttaaaggtgttcacactataaaagcatatacgatgtg
atggtatttgatggagcgtatattgtatcaggtatttccgttggatacgaattattcgtacgaccctcat
agtttaaactatcagtgtttgacaggatatattggcgggtaaacctaagagaaaagagcgtttattagaa
taacggatatttaaaagggcgtgaaaaggtttatccgttcgtccatttgtatgtgcatgccaaccacagg
gttcccctcgggatcaaagtactttgatccaacccctccgctgctatagtgcagtcggcttctgacgttc
agtgcagccgtcttctgaaaacgacatgtcgcacaagtcctaagttacgcgacaggctgccgccctgccc
ttttcctggcgttttcttgtcgcgtgttttagtcgcataaagtagaatacttgcgactagaaccggagac
attacgccatgaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccgacgaccagga
cttgaccaaccaacgggccgaactgcacgcggccggctgcaccaagctgttttccgagaagatcaccggc
accaggcgcgaccgcccggagctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtga
ccaggctagaccgcctggcccgcagcacccgcgacctactggacattgccgagcgcatccaggaggccgg
cgcgggcctgcgtagcctggcagagccgtgggccgacaccaccacgccggccggccgcatggtgttgacc
gtgttcgccggcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcgggcgcgaggccg
ccaaggcccgaggcgtgaagtttggcccccgccctaccctcaccccggcacagatcgcgcacgcccgcga
gctgatcgaccaggaaggccgcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctg
taccgcgcacttgagcgcagcgaggaagtgacgcccaccgaggccaggcggcgcggtgccttccgtgagg
acgcattgaccgaggccgacgccctggcggccgccgagaatgaacgccaagaggaacaagcatgaaaccg
caccaggacggccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatcgcggccgggt
acgtgttcgagccgcccgcgcacggctcaaccgtgcggctgcatgaaatcctggccggtttgtctgatgc
caagctggcggcctggccggccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgt
gtatttgagtaaaacagcttgcgtcatgcggtcgctgcgtatatgatgcgatgagtaaataaacaaatac
gcaaggggaacgcatgaaggttatcgctgtacttaaccagaaaggcgggtcaggcaagacgaccatcgca
acccatctagcccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgatccccagggca
gtgcccgcgattgggcggccgtgcgggaagatcaaccgctaaccgttgtcggcatcgaccgcccgacgat
tgaccgcgacgtgaaggccatcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggac
ttggctgtgtccgcgatcaaggcagccgacttcgtgctgattccggtgcagccaagcccttacgacatat
gggccaccgccgacctggtggagctggttaagcagcgcattgaggtcacggatggaaggctacaagcggc
ctttgtcgtgtcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgctggccgggtac
gagctgcccattcttgagtcccgtatcacgcagcgcgtgagctacccaggcactgccgccgccggcacaa
ccgttcttgaatcagaacccgagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatc
aaaactcatttgagttaatgaggtaaagagaaaatgagcaaaagcacaaacacgctaagtgccggccgtc
cgagcgcacgcagcagcaaggctgcaacgttggccagcctggcagacacgccagccatgaagcgggtcaa
ctttcagttgccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaagaccattacc
gagctgctatctgaatacatcgcgcagctaccagagtaaatgagcaaatgaataaatgagtagatgaatt
ttagcggctaaaggaggcggcatggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgt
gtggaggaacgggcggttggccaggcgtaagcggctgggttgtctgccggccctgcaatggcactggaac
ccccaagcccgaggaatcggcgtgacggtcg
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