Mycoplasmoides genitalium G37生殖支原体标准菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Mycoplasmoides genitalium G37生殖支原体标准菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Name:
Mycoplasmoides genitalium (Tully et al. 1983) Gupta et al. 2018
Synonym(s):
Mycoplasma genitalium Tully et al. 1983
NTCC No.:
NTCC19775, Type strain
Strain designation:
G37
Isolated from:
urethra of male with non-gonococcal urethritis
Country:
United States of America
Date of sampling:
before 16.10.2007
History:
<- J. K. Davis {2007} <- J. G. Tully; G37
Genbank accession numbers:
complete genome: L43967
16S rRNA gene: X77334
Cultivation conditions:
Medium SP4-Z MEDIUM + glucose, + 5% CO2, 37°C
Incubation time: 1-2 days

SP4-Z MEDIUM
The difference between this medium and "normal" SP4 medium (Medium 1076) is that the
yeast extract was left out and DNA was added instead. Since most mycoplasmas use either
glucose or arginine as their energy source one is added to the media. Do not combine both
in one medium, arginine can inhibit the growth of some glucose utilizers (unknown
reason).
For 200 ml of medium
PPLO broth 0.70 g
Tryptone 2.00 g
Bacto-Peptone (BD) 1.00 g
distilled water 130.00 ml
DNA (fish sperm; SERVA) 0.04 g
Adjust pH to 7.4; autoclave at 121C for 15 minutes.
Add aseptically:
CMRL-1066 medium (10x concentrated; GIBCO) 10 ml
Yeastolate (BD; 2%, autoclaved) 20 ml
Fetal bovine serum (heat-inactivated) 17 ml
Swine serum (heat-inactivated) 17 ml
As energy source add either
Glucose (50% aqueous solution) 2 ml
or
Arginine HCl (50% aqueous solution) 2 ml
For Ureaplasma add instead of Glucose or Arginine
Urea 0.4 g
For agar plates use only 126 ml of distilled water and add 2 g of agar. Medium containing
agar should be placed at 55C before adding supplements.
Summary and
additional information:
<- J. K. Davis {2007} <- J. G. Tully; G37. Urethra of male with non-gonococcal urethritis; United States of America; sampling date: before 16.10.2007. Type strain. Taxonomy/description (21706). Sequence accession no. complete genome: L43967, 16S rRNA gene: X77334. (Medium 1076b + glucose, 37°C, + 5% CO2).
Literature:
Tully, J. G. (1983). Mycoplasma genitalium, a new species from the human urogenital tract.Int.J.Syst.Bacteriol. 33 : 387-396 .

Risk group:
2


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