BA/F3_EGFR L858R-T790M-C797S cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

BA/F3_EGFR L858R-T790M-C797S cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

Cell Line Name：

Ba/F3_EGFR-L858R/T790M/C797S

Gene Synonyms:

Epidermal Growth Factor Receptor, ERBB1,EGFR

Host Cell:

Ba/F3

Stability:16passages

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10% FBS+1 ug/ml puromycin

Mycoplasma Status:

Negative

II.Background

EGFR is widely recognized for itsimportance in cancer. Amplification and mutations have been shown to be drivingevents in many cancer types. Its role in non-small cell lung cancer,glioblastoma and basal-like breast cancers has spurred many research and drugdevelopment efforts. Tyrosine kinase inhibitors have shown efficacy in EGFRamplfied tumors, most notably gefitinib and erlotinib. Mutations in EGFR havebeen shown to confer resistance to these drugs, particularly the variant T790M,which has been functionally characterized as a resistance marker for both ofthese drugs. The later generation TKI's have seen some success in treatingthese resistant cases, and targeted sequencing of the EGFR locus has become acommon practice in treatment of non-small cell lung cancer. Overproduction ofligands is another possible mechanism of activation of EGFR. ERBB ligands includeEGF, TGF-a, AREG, EPG, BTC, HB-EGF, EPR and NRG1-4 (for detailed informationplease refer to the respective ligand section). In ligand-activated cancers,Cetuximab appears to be more effective than tyrosine-kinase inhibitors.

III. Representative Data

1. WB of EGFR-L858R/T790M/C797S expression

Figure 1. WB of EGFR Expression

Lane 1: Negative control

Lane 2: EGFR-WT

Lane 3: EGFR-L858R/T790M

Lane 4: EGFR-L858R/T790M/C797S

2. Sanger sequencing

Figure 2. Sanger Sequencing ofEGFR-L858R/T790M/C797S

3. Anti-proliferation assay

Figure 3. Anti-proliferation assay of threereference compounds on the Ba/F3_EGFR-L858R/T790M/C797S Stable Cell Line

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