pX8dT plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pX8dT plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
As vector backbones, we used a pEXR vector (∼2.6 kbp) to minimize the lengths of T7 plasmids carrying medium- and small-sized gene segments (VP6, VP7, and NSP1-NSP5 genes) and a traditional pX8dT vector (∼3.0 kbp) (34) to carry large-sized gene segments (VP1-VP3 genes). As T7 expression plasmids, we used a pEX-A2J1-derived pEXR vector that carries HDV ribozyme and T7 RNA polymerase terminator sequences (artificially synthesized by Eurofins Genomics) (for VP6, VP7, and NSP1-NSP5 genes) and a modified pX8dT vector (34) (for VP1-VP3 genes). pX8dT plasmid is a derivative of pBluescriptII (Stratagene) from which a BssHII/ClaI fragment of the multiple cloning site containing the original T7 promoter was deleted. It contains the 84 base HDV antigenomic ribozyme sequence in the SmaI site followed immediately by a T7 transcription terminator sequence cloned in the BamHI site.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net