SET-2 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

SET-2 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
SET-2 Cells
Cat No.: NTCC-ACC608

Cell line:
SET-2
NTCC no.:
NTCC-ACC608
Species:
human (Homo sapiens)
Cell type:
essential thrombocythemia
Origin:
established from the peripheral blood of a 71-year-old woman with essential thrombocythemia at megakaryoblastic leukemic transformation in 1995; cells were described in the literature to carry the JAK2 V617F mutation
Biosafety level:
1
Permissions and restrictions:
A

Cell Culture Data:
Morphology:
mostly single cells in suspension, some cells grow loosely adherent, 1-3% giant cells; image;

Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
start and cultivate in 24- or 12-well plates for optimal growth; seed out at ca. 0,8 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split culture 1:2 every 3-4 days; possibly no cell growth during first week after thawing
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-80 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD7 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD38 +, CD42 -, CD71 +, HLA-DR +; image
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human flat-moded near-diploid karyotype with 8% polyploidy; 46-47<2n>XX, +13, -14, +21, +mar, add(4)(p15), der(5)t(5;19)(q11;q13), der(7)t(7;14)(q31;q11), del(9)(p11p24)ins(9;?21)(p11;p11p12), add(9)(q11)t(9;22)(p24;q12), der(13)del(13)(q11-q13)t(13;21)(q34;q11-12), der(13)t(13;21)(q34;q11-12), ins(16;9)(q22;?p23p24), der(19)t(9;19)(p24;q13), ider(?21;9)(p10p13;p24), der(21)r(21;9)(p1?1q1?1;p24p2?3); carries multiple rearrangements effecting 9p24 amplification; resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

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