pRU1064等plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pRU1064等plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
E. coli DH5a T-1 F2 w80lacZDM15 D(lacZYA-argF) U169 recA1 endA1 hsdR17(r{
k , mz
k )
phoA supE44 thi-1 gyrA96 relA1 tonA
Invitrogen
R. leguminosarum
3841 Str derivative of R. leguminosarum biovar viciae strain 300 Johnston & Beringer (1975)
RU1416 3841 containing pJP2 This work
RU1683–RU1692 3841 with plasmids pRU1119–pRU1128 containing dctAp This work
RU1708 3841 containing pRU1140 This work
RU1709 3841 containing pRU1141 This work
RU1712 3841 containing pRU1147 This work
RU1713 3841 containing pRU1148 This work
RU1715 3841 containing pRU1156 This work
RU1716 3841 containing pRU1157 This work
RU1724 3841 containing pRU1164 This work
RU1725 3841 containing pRU1161 This work
RU1728 3841 containing pRU1167 This work
Plasmids
pCR2.1TOPO Apr
, Kmr
; PCR product cloning vector Invitrogen
pOT2 Gmr
; promoter probe vector with promoterless gfpUV Allaway et al. (2001)
pJP2 Tcr
; stable broad-host-range cloning vector Prell et al. (2002)
pBJA27 Apr
; gfpmut3.1 Anderson et al. (1998)
pBJA110 Apr
; gfpmut3.1 (LAA)
pBJA111 Apr
; gfpmut3.1 (LVA)
pBJA112 Apr
; gfpmut3.1 (AAV)
pBJA113 Apr
; gfpmut3.1 (ASV)
DsRed2.0 Apr
; dsRed2.0 from Discosoma Clontech
DsRedT.3, DsRedT.4 Apr
; fast-maturing versions of dsRed2.0 Bevis & Glick (2002)
mRFP1 Apr
; mRFP1 from Discosoma in pRSETB Campbell et al. (2002)
pMN402 Hygromycinr
; gfp+ Scholz et al. (2000)
pMP220 Tcr
; IncP broad-host-range mobilizable promoter probe vector employing
E. coli lacZ as reporter gene
Spaink et al. (1987)
pRK2013 Kmr
; ColEI replicon with RK2 tra genes, helper plasmid used for
mobilizing plasmids
Figurski & Helinski (1979)
pRSETB Apr
; T7 expression vector Invitrogen
pRU491 Gmr
; SpeI–HindIII fragment containing the dpp promoter from R.
leguminosarum strain 3841
This work
pRU604 Gmr
; PmeI–HindIII fragment containing the xylose kinase promoter This work
pRU977 pRU1701 containing the dctA promoter This work
pRU1064 Tcr
; HindIII–SacI fragment containing gfpUV from pOT2 cloned into pJP2 This work
pRU1097 Gmr
; p318/p319 PCR product (gfpmut3.1) from pBJA27 cloned in pOT2
as SpeI–SacI
This work
pRU1098 Gmr
; p318/p320 PCR product; (gfpmut3.1 LAA) from pBJA110 cloned in
pOT2 as SpeI–SacI
This work
pRU1099 Gmr
; p318/p321 PCR product (gfpmut3.1 LVA) from pBJA111 cloned in
pOT2 as SpeI–SacI
This work
pRU1100 Gmr
; p318/p322 PCR product (gfpmut3.1 AAV) from pBJA112 cloned in
pOT2 as SpeI–SacI
This work
pRU1101 Gmr
; p318/p323 PCR product (gfpmut3.1 ASV) from pBJA113 cloned in
pOT2 as SpeI–SacI
This work
pRU1102 Gmr
; p201/p203 PCR product (gusA) from pJP2 cloned in pOT2 as
SpeI–SacI
pRU1103 Gmr
; p348/p349 PCR product (lacZ) from pMP220 cloned in pOT2 as
SpeI–XhoI
This work
pRU1104 Gmr
; p346/p347 PCR product (DsRed2.0) from DsRed2.0 cloned in pOT2
as SpeI–SacI
This work
pRU1105 Gmr
; p385/p386 PCR product (DsRedT.3) from DsRedT.3 cloned in pOT2
as SpeI–SacI
This work
pRU1106 Gmr
; p385/p386 PCR product (DsRedT.4) from DsRedT.4 cloned in pOT2
as SpeI–SacI
This work
pRU1119–pRU1128 Gmr
; dctA promoter from pRU977 cloned in pRU1097–pRU1106, respectively,
using SphI–SstII sites
This work
pRU1140 Gmr
; dpp promoter from pRU491 cloned in pRU1104 as SpeI–HindIII sites This work
pRU1141 Gmr
; dpp promoter from pRU491 cloned in pRU1105 as SpeI–HindIII sites This work
pRU1144 Gmr
; p408/p409 PCR product (mRFP1) from pRSETB cloned in pOT2 as
SpeI–SacI
This work
pRU1147 Tcr
; xylose promoter from pRU604 cloned in pRU1144 using PmeI–HindIII
sites
This work
pRU1148 Gmr
; dpp promoter from pRU491 cloned in pRU1144 as SpeI–HindIII sites This work
pRU1156 Tcr
; HindIII–SacI fragment containing gfpmut3.1 from pRU1097 cloned into
pJP2
This work
pRU1157 Tcr
; xylose promoter from pRU604 cloned in pRU1156 using PmeI–HindIII
sites
This work
pRU1161 Tcr
; HindIII/SacI fragment containing mRFP1 from pRU1144 cloned into pJP2 This work
pRU1164 Tcr
; dpp promoter from pRU491 cloned in pRU1161 as SpeI–HindIII sites This work
pRU1167 Tcr
; xylose promoter from pRU604 cloned in pRU1064 using PmeI–HindIII
sites
This work
pRU1701 Gmr
; promoter probe vector with promoterless gfp+ Hosie et al. (2002)
pRU1716 Gmr
; SphI–SstII fragment containing dctA promoter in pOT2 Poole et al. (1994b)
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