KARPAS-422 cell line弥漫大B细胞淋巴瘤细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

KARPAS-422 cell line弥漫大B细胞淋巴瘤细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心
KARPAS 422
Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 06101702
Cell Line Name: KARPAS 422
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: KARPAS 422 (ECACC 06101702)
Keywords: Human B cell non-Hodgkin lymphoma
Cell Line Description: Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma (intra-abdominal, diffuse large cell lymphoma, refractory, terminal). Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).
Species: Human
Tissue of Origin: B cell lymphoma
CellType: Round polygonal cells growing singly or in small clusters in suspension
Growth Mode: Suspension
Karyotype: Hyperdiploid with 10% polyploidy
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube; slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%.Maintain cultures between 5 - 20 x100,000 cells/ml by splitting saturated cultures 1:2 every 2-4 days; 5% CO2; 37°C. Doubling time approximately 60-90 hours. Round polygonal cells growing singly or in small clusters in suspension.
Culture Medium: RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
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