Raji cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：Raji cell line细胞株细胞系
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Raji cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Culture Type: Cell line
Catalogue No.: 85011429
Cell Line Name: RAJI
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: RAJI (ECACC 85011429)
Keywords: Human Burkitt's lymphoma
Cell Line Description: Established in 1963 from Burkitt's lymphoma in an 11 year old male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a 'non-producer'; the EBV genome carries deletions attributed to preventing the formation of virus particles. Ethnicity: Black.
Species: Human
Tissue of Origin: lymph
CellType: Lymphoblastoid
Growth Mode: Suspension
DNA Profile: STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 16,19
Karyotype: 2n = 46, diploid
Subculture Routine: If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, and then slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of approximately 5 x 100,000 cells/ml into fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily and keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up 3-4 days. Once the culture is established the serum concentration can be reduced to 10%. Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
Culture Type: Cell line
Catalogue No.: 85011429
Cell Line Name: RAJI
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: RAJI (ECACC 85011429)
Keywords: Human Burkitt's lymphoma
Cell Line Description: Established in 1963 from Burkitt's lymphoma in an 11 year old male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a 'non-producer'; the EBV genome carries deletions attributed to preventing the formation of virus particles. Ethnicity: Black.
Species: Human
Tissue of Origin: lymph
CellType: Lymphoblastoid
Growth Mode: Suspension
DNA Profile: STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 16,19
Karyotype: 2n = 46, diploid
Subculture Routine: If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, and then slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of approximately 5 x 100,000 cells/ml into fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily and keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up 3-4 days. Once the culture is established the serum concentration can be reduced to 10%. Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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