pTRKH3-ldhGFP plasmid vector乳酸菌表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pTRKH3-ldhGFP plasmid vector乳酸菌表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

Vector backbone pTRKH3
(Search Vector Database)
Backbone size w/o insert (bp) 7493
Vector type Bacterial Expression
GROWTH IN BACTERIA
Bacterial Resistance(s) erythromycin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number Low Copy
GENE/INSERT
Gene/Insert name erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
Alt name Enterococcus faecalis ermB promoter
Alt name synthetic construct mgfp5
Species Enterococcus faecalis and synthetic construct
Insert Size (bp) 1279
Mutation presence of an additional 183-bp region at N terminal on insert
CLONING INFORMATION
Cloning method Restriction Enzyme
5′ cloning site SalI (not destroyed)
3′ cloning site BamHI (not destroyed)
5′ sequencing primer AGTGAAAAGTTCTTCTCCTT
3′ sequencing primer GCGGCACGACTTCTTCAAGAGC
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net