U-87 MG-luc cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

U-87 MG-luc cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
U-87 MG-luc is a luciferase expressing cell line which was stably transfected with firefly luciferase gene (luc). The
cell line was established by transducing lentivirus containing luciferase 2 gene under the control of human ubiquitin
C promoter. This cell line can be used in vivo to establish:
• Subcutaneous tumor models
• Orthotopic tumor model (Intracerebral implantation)

General Information
Bioware Ultra Cell Line U-87 MG-luc
Designation U-87 MG-luc
Tissue Human: glioblastoma; Brain
Source of Parental Line ATCC (HTB-14™)
Gene Transfer Vehicle pGL4 luc Lentivirus
Bioluminescence In Vitro Approximately 1250 photons/sec/cell. Exact number will vary depending
on imaging and culturing conditions.
Recommended Media Eagle's Minimum Essential Medium (ATCC Catalog No. 30-2003)
Supplement the above with Heat inactivated FBS at final concentration 10%
Cell Doubling Time 16 hr
Other Recommendations When initially thawing, use T25 flask. Cells should be ready to expand next day.
We recommend not using any antibiotics in the media.


References
1. Cuthbert and Lipsky (1997) Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Research 37:3498-3505.
2. Sherley, Stadler, and Stadler (1995) A quantitative method for the analysis of mammalian cell proliferation in culture in terms of dividing and non-dividing cells. Cell Proliferation 28:137-144.

【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net