KELLY cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

KELLY cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:
KELLY
Cat no.:
ACC 355
Species:
human (Homo sapiens)
Cell type:
neuroblastoma
Origin:
established from a patient with neuroblastoma; ca. 100-fold genomic amplification of NMYC in der(17)hsr
Reference(s):
14858, 14947
Biosafety level:
1
Permissions and restrictions:
A

Cell Culture Data:
Morphology:
adherent, round to fusiform with polar neuritic processes, growing in mono- and multilayers;

Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split confluent culture 1:3 to 1:10 every 3-6 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 25-30 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:
Mycoplasma:
contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament +, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, LDH, MDH
Cytogenetics:
human near-diploid karyotype with 20% polyploidy - 46-47<2n>X, -7, -8, +16, +22, +2mar, del(1)(p34), t(2;7)(p12;q11), add(4)(p15.3), add(5)(p15), add(9)(q34), add(10)(q24), add(11)(q24), add(14)(q32), ins(17;2)(q21;p24 hsr)x2 - del(1p34) and 2p24 hsr are typical of late-stage neuroblastoma - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV -


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