- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pOPINEneo,
Plasmid 53536.
pOPINEneo多系统多功能表达载体,适用于原核、真核、昆虫表达.
pTriex-based expression vector which adds a C-his8 tag to the inserted gene
BACKBONE
- Vector backbonepTriex-2
- Backbone manufacturerEMDMillipore
Backbone size (bp)7049
- Vector typeMammalian Expression, Bacterial Expression, Insect Expression
GROWTH IN BACTERIA
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)DH5alpha
- Growth instructionsnone
- Copy numberHigh Copy
>pOPINEneo sequence 7049 bps
tcctgcatcttttaatcaaatcccaagatgtgtataaacgcgccggtatgtacaggaagaggtttatact
aaactgttacattgcaaacgtggtttcgtgtgccaagtgtgaaaaccgatgtttaatcaaggctctgacg
catttctacaaccacgactccaagtgtgtgggtgaagtcatgcatcttttaatcaaatcccaagatgtgt
ataaaccaccaaactgccaaaaaatgaaaactgtcgacaagctctgtccgtttgctggcaactgcaaggg
tctcaatcctatttgtaattattgaataataaaacaattataaatgtcaaatttgttttttattaacgat
acaaaccaaacgcaacaagaacatttgtagtattatctataattgaaaacgcgtagttataatcgctgag
gtaatatttaaaatcattttcaaatgattcacagttaatttgcgacaatataattttattttcacataaa
ctagacgccttgtcgtcttcttcttcgtattccttctctttttcatttttctcttcataaaaattaacat
agttattatcgtatccatatatgtatctatcgtatagagtaaattttttgttgtcataaatatatatgtc
ttttttaatggggtgtatagtaccgctgcgcatagtttttctgtaatttacaacagtgctattttctggt
agttcttcggagtgtgttgctttaattattaaatttatataatcaatgaatttgggatcgtcggttttgt
acaatatgttgccggcatagtacgcagcttcttctagttcaattacaccattttttagcagcaccggatt
aacataactttccaaaatgttgtacgaaccgttaaacaaaaacagttcacctcccttttctatactattg
tctgcgagcagttgtttgttgttaaaaataacagccattgtaatgagacgcacaaactaatatcacaaac
tggaaatgtctatcaatatatagttgctgatggccggccgtaatgagacgcacaaactaatatcacaaac
tggaaatgtctatcaatatatagttgctctagttattaatagtaatcaattacggggtcattagttcata
gcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgaccc
ccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaa
tgggtggactatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgcccc
ctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcc
tacttggcagtacatctacgtattagtcatcgctattaccatgcatggtcgaggtgagccccacgttctg
cttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgt
gcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcg
gggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgag
gcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgc
cccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacagg
tgagcgggcgggacggcccttctccttcgggctgtaattagcgcttggtttaatgacggcttgtttcttt
tctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggctg
tccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggc
ggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctgg
ttattgtgctgtctcatcattttggcaaagaattggatcggaccgaaattaatacgactcactatagggg
aattgtgagcggataacaattccccggagttaatccgggacctttaattcaacccaacacaatatattat
agttaaataagaattattatcaaatcatttgtatattaattaaaatactatactgtaaattacattttat
ttacaatcaaaggagatataccatggcacaccatcaccaccatcacagcagcggtaccgtcgacccgact
ggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttaca
ctttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctat
gaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaa
cttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcc
cttcccaacagttgcgcagcctgaatggcgaatggcgccaagctttctagaccagtttaaacatcaccat
caccatcaccatcactgaattctcagatctcggcgcgcctgcaggtcgacggtaccggttcgaagcttgc
ggccgcacagctgtatacacgtgcaagccagccagaactcgccccggaagaccccgaggatctcgagcac
caccatcaccatcaccatcactaagtgattaacctcaggtgcaggctgcctatcagaaggtggtggctgg
tgtggtaattccggttattttccaccatattgccgtcttttggcaatgtgagggcccggaaacctggccc
tgtcttcttgacgagcattcctaggggtctttcccctctcgccaaaggaatgcaaggtctgttgaatgtc
gtgaaggaagcagttcctctggaagcttcttgaagacaaacaacgtctgtagcgaccctttgcaggcagc
ggaaccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacacctgcaaaggc
ggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatggctcacctcaagcgta
ttcaacaaggggctgaaggatgcccagaaggtaccccattgtatgggatctgatctggggcctcggtgca
catgctttacatgtgtttagtcgaggttaaaaaaacgtctaggccccccgaaccacggggacgtggtttt
cctttgaaaaacacgatgataagcttgccacaacccgcgataattcctgcagccaatatgggatcggcca
ttgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggc
acaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttcttttt
gtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggcca
cgacgggcgttccttgcgcagcagtgctcgacgttgtcactgaagcgggaagggactggctgctattggg
cgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgat
gcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcg
agcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggct
cgcgccagccgaactgttcgccaggctcaaggcgcgtatgcccgacggcgatgatctcgtcgtgactcat
ggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggc
tgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga
atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgc
cttcttgacgagttcttctgaggggatcaattctctagccaatgccctggctcacaaataccactgagat
cgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaa
taaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatg
ggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgta
actagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaagcatgcggaggaaat
tctccttgaagtttccctggtgttcaaagtaaaggagtttgcaccagacgcacctctgttcactggtccg
gcgtattaaaacacgatacattgttattagtacatttattaagcgctagattctgtgcgttgttgattta
cagacaattgttgtacgtattttaataattcattaaatttataatctttagggtggtatgttagagcgaa
aatcaaatgattttcagcgtctttatatctgaatttaaatattaaatcctcaatagatttgtaaaatagg
tttcgattagtttcaaacaagggttgtttttccgaaccgatggctggactatctaatggattttcgctca
acgccacaaaacttgccaaatcttgtagcagcaatctagctttgtcgatattcgtttgtgttttgttttg
taataaaggttcgacgtcgttcaaaatattatgcgcttttgtatttctttcatcactgtcgttagtgtac
aattgactcgacgtaaacacgttaaatagagcttggacatatttaacatcgggcgtgttagctttattag
gccgattatcgtcgtcgtcccaaccctcgtcgttagaagttgcttccgaagacgattttgccatagccac
acgacgcctattaattgtgtcggctaacacgtccgcgatcaaatttgtagttgagctttttggaattgcg
atcgcataacttcgtatagcatacattatacgaagttataagctcggaacgctgcgctcggtcgttcggc
tgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcagg
aaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttc
cataggctccgccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagg
actataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgctt
accggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatc
tcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctg
cgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcc
actggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaact
acggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagt
tggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagatt
acgcgcagaaaaaaaggatctcaagaagatcctttgttaccaatgcttaatcagtgaggcacctatctca
gcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagg
gcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagc
aataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtct
attaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattg
ctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaag
gcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcaga
agtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccat
ccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgacc
gagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatc
attggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaac
ccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacagg
aaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttccttttt
caatattattgaagcatttatcagggttattgtctcatgtccgcgcgtt
CLONING INFORMATION
Cloning methodLigation Independent Cloning
- 公告/新闻
