pUR4547抗体酵母展示载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥99865
- 货 号：pUR4547抗体酵母展示载体质粒
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pUR4547抗体酵母展示载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
For secretion by S. cerevisiae, the fragments were recloned in episomal vector pUR4547 (Fig. 1B), which is identical to previously described pUR4548 (10) but does not encode any tag sequences. The host strain used, VWK18gal1, was a gal1 derivative of CEN.PK102-3A (MATa leu2 ura3) obtained by disruption of the GAL1 gene by integration of the S. cerevisiae URA3 gene (40) at this locus. Production on a 0.5-liter (shake flasks) or a 10-liter scale was performed at BAC B.V. (Naarden, The Netherlands) as described previously (43). The VHH fragments were purified by ion-exchange chromatography with Mono-S-Sepharose (Pharmacia) after concentrating the culture supernatant by ultrafiltration.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
For secretion by S. cerevisiae, the fragments were recloned in episomal vector pUR4547 (Fig. 1B), which is identical to previously described pUR4548 (10) but does not encode any tag sequences. The host strain used, VWK18gal1, was a gal1 derivative of CEN.PK102-3A (MATa leu2 ura3) obtained by disruption of the GAL1 gene by integration of the S. cerevisiae URA3 gene (40) at this locus. Production on a 0.5-liter (shake flasks) or a 10-liter scale was performed at BAC B.V. (Naarden, The Netherlands) as described previously (43). The VHH fragments were purified by ion-exchange chromatography with Mono-S-Sepharose (Pharmacia) after concentrating the culture supernatant by ultrafiltration.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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