pGreenFire1-ISRE+EF1a-Puro BioVector NTCC质粒载体菌种细胞基因保藏中心

pGreenFire1-ISRE+EF1a-Puro BioVector NTCC质粒载体菌种细胞基因保藏中心
pGreenFire1-ISRE+EF1a-Puro信号转导途径实时荧光检测载体，共表达dscGFP和Luc荧光来研究ISRE信号转导，puro细胞筛选标记。
Monitor signal transduction in real time

With the line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-ISRE (EF1α-puro) Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from ISRE transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of ISRE-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to ISRE activity. The result is the ability to quantitatively measure ISRE activity by fluorescence and luciferase activity.
Sort responsive cells with dscGFP
Measure activity with luciferase
Leverage SBI’s highly-regarded lentivectors
Create stable signaling pathway reporter cell lines
Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGreenFire1-ISRE (EF1α-puro) Lentivector

To facilitate cell line construction, the pGreenFire1-ISRE (EF1α-puro) Lentivector comes with a constitutively-expressed puromycin marker from the EF1α promoter. Or you can choose a neomycin marker (EF1α-neo; Cat.# TR016PA-N) or no marker at all (Cat.# TR016PA-1). In addition, all forms of this vector are available as both lentivector plasmid, and pre-packaged virus.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net