Brevundimonas diminuta strain缺陷假单胞菌菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心

Brevundimonas diminuta strain缺陷假单胞菌菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心
Brevundimonas diminuta
Cat No.: 19146

Designation: FDA strain PCI 818 [CCUG 24715, DSM 1635, LMG 10743]
Deposited Name: Pseudomonas sp.
Product Description: Formerly Pseudomonas diminuta. Used for sterility assurance and membrane filter testing.

Medium
Medium 2495: 10mM Phosphate Buffer
Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 30°C
Atmosphere: Aerobic
Propagation Procedure
1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Rehydrate the entire pellet with approximately 0.5 mL of #2495 broth. Aseptically transfer the entire
contents to a 56
mL tube of #2495 broth. Additional test tubes can be inoculated by transferring 0.5
mL of the primary broth tube to these secondary tubes.
3. Use several drops of the primary broth tube to inoculate a #3 plate and/or #3 agar slant.
4. Incubate at 30°C for 48 to 72 hours.

Phosphate buffer is for rehydration and transfer only, not for growth.
Two colony types may be found in this strain if it is passed through Nutrient Broth (BD 234000).
To prevent proliferation of the second colony type, rehydrate the vial as above and maintain the culture by
transferring from agar to agar.
Purified genomic DNA of this strain is available

【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net