Rosetta 2(DE3) strain菌株菌种-感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

Rosetta 2(DE3) strain菌株菌种-感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心
A common method for transformation of DNA plasmids into E. coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Biovector prepared competent cells. Biovector competent cells represent the widest selection available for protein expression. Every Biovector competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency. T7 expression strains are lysogens of bacteriophage DE3, as indicated by the (DE3). These hosts carry a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in appropriate T7 expression vectors, using IPTG as an inducer.
Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE2 (CamR)

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