EOL-1 cell line人白血病细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

EOL-1 cell line人白血病细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Product Sheet
EOL-1 cell line
Cat No.: NTCC592181

Cell line:
EOL-1
NTCC no.:
ACC 386
Species:
human (Homo sapiens)
Cell type:
acute myeloid (eosinophilic) leukemia
Origin:
established in 1984 at diagnosis from the peripheral blood of a 33-year-old man with acute myeloid (eosinophilic) leukemia (AML) following hypereosinophilic syndrome; we found that EOL-1 carries a KMT2A (MLL) partial tandem duplication; described in the literature to carry the fusion FIP1L1-PDGFRA
Reference(s):
14439, 14751, 14868, 14912
Biosafety level:
1
Permissions and restrictions:
A

NTCC Cell Culture Data:
Morphology:
(relatively small) round cells growing in suspension, singly or in small clusters; image; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; start culture with 20% FBS; maintain at 0.5-2.0 x 106 cells/ml; split 1:2 to 1:3 every 3-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60 hours
Harvest:
maximum density of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR +; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
originally confirmed as human with IEF of LDH, MDH, NP
Cytogenetics:
human hyperdiploid karyotype with 7.5% polyploidy - 50(48-51)<2n>,XY, +4, +6, +8, +19, del(4)(q12)x2, del(9)(q22) - del(4) effects cryptic FIP1L1-PDGFRA fusion - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of the Biosafety in Microbiological and Biomedical Laboratories from the U.S. Department of Health and Human Services Centers for Disease Control and Prevention and National Institutes for Health.



【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net