pMXs等逆转录病毒表达载体包装体系质粒和细胞选择方法 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥19865
- 货 号：pMXs等逆转录病毒表达载体包装体系质粒和细胞选择方法
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pMXs等逆转录病毒表达载体包装体系质粒和细胞选择方法 BioVector NTCC质粒载体菌种细胞基因保藏中心
A: Retrovirus can be packaged using 293T or 293RTV cells. We also offer Platinum Packaging Cell Lines that can be used to package either amphotropic, ecotropic, or pantropic retrovirus. Here are the options for making retrovirus:
1. Transfect a Platinum retrovirus packaging cell line, such as Plat-A (amphotropic) or Plat-E (ecotropic), which stably expresses both MMLV Gag-Pol and an amphotropic or ecotropic envelope protein, with a retroviral expression vector. These cells require transfection of only an expression vector to produce retrovirus. Unlike VSVG pseudotyped virus, amphotropic and ecotropic viruses are not very stable, and can't handle ultracentrifugation.
2. Cotransfect the Plat-GP Retroviral Packaging Cell Line, which stably expresses MMLV Gag-Pol, with pCMV-VSVG and an expression vector. This cell line produces VSVG pseudotyped retrovirus that can infect any cell type.
3. Cotransfect 293T or 293RTV cells with 3 vectors:
a. pCMV-Gag-Pol vector
b. Envelope vector, either pCMV-VSVG, pCMV-Ampho, or pCMV-Eco
c. A Retroviral Expression Vector
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
A: Retrovirus can be packaged using 293T or 293RTV cells. We also offer Platinum Packaging Cell Lines that can be used to package either amphotropic, ecotropic, or pantropic retrovirus. Here are the options for making retrovirus:
1. Transfect a Platinum retrovirus packaging cell line, such as Plat-A (amphotropic) or Plat-E (ecotropic), which stably expresses both MMLV Gag-Pol and an amphotropic or ecotropic envelope protein, with a retroviral expression vector. These cells require transfection of only an expression vector to produce retrovirus. Unlike VSVG pseudotyped virus, amphotropic and ecotropic viruses are not very stable, and can't handle ultracentrifugation.
2. Cotransfect the Plat-GP Retroviral Packaging Cell Line, which stably expresses MMLV Gag-Pol, with pCMV-VSVG and an expression vector. This cell line produces VSVG pseudotyped retrovirus that can infect any cell type.
3. Cotransfect 293T or 293RTV cells with 3 vectors:
a. pCMV-Gag-Pol vector
b. Envelope vector, either pCMV-VSVG, pCMV-Ampho, or pCMV-Eco
c. A Retroviral Expression Vector
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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