H1299 cell line stably expressing DSRED Labeled Actin红色荧光稳转细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥99835
- 货 号：H1299 cell line stably expressing DSRED Labeled Actin红色荧光稳转细胞株
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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H1299 cell line stably expressing DSRED Labeled Actin红色荧光稳转细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Product: H1299 cell line stably expressing DSRED Labeled Actin.
Description: This product is a cell line stably expressing the Actin. Actins are highly conserved proteins that are involved in various types of cell motility. This cell line also expresses DSRED protein and the puromycin resistance gene.
Source of parental line: H1299 Organism: Homo sapiens, human Tissue: lung Cell type: Epithelial
Thawing procedure:
The vial of cells should be thawed in a 37oC water bath with gentle agitation. For optimal performance, the vial should be thawed in under two minutes. Ensure that the cap of the vial did not loosen upon thawing, and re‐tighten as needed. Spray the vial with 70% EtOH and wipe off. Repeat. Using aseptic technique, add the contents of the vial to 9 ml of complete growth medium (without selection). Centrifuge for 5 min. at 125 x g. Aspirate the medium, being careful not to disturb the pellet. Resuspend in 10 mL of complete growth medium, and place into a culture vessel of your choice. Only add selection to the medium after 24 hours in culture.
Culture conditions:
Complete Growth Medium The base medium for this cell line is DMEM. For optimal growth and maintenance of selection, add the following components to the base medium: dialyzed fetal bovine serum to a final concentration of 10%. Selection Puromycin to a final concentration of 2 µg/mL
Culture temperature: 37 0 C with 5% CO2
Subculture:
Replace culture medium with selection‐free medium and incubate for up to 6 hours. Rinse the cells with PBS without cations, digest cells with 0.25% (w/v) Trypsin‐EDTA (0.53 mM)solution and split at 1:3 to 1:10 ratio.
Cryopreservation: Freeze slowly in complete growth medium supplemented with 5% (v/v) DMSO.
Mycoplasma: Negative
Fluorescence: Image of H1299/DSRED Labeled Actin Cell Line. Observing the cells climbing to the carry sheet glass, which are stained with DAPI.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
Product: H1299 cell line stably expressing DSRED Labeled Actin.
Description: This product is a cell line stably expressing the Actin. Actins are highly conserved proteins that are involved in various types of cell motility. This cell line also expresses DSRED protein and the puromycin resistance gene.
Source of parental line: H1299 Organism: Homo sapiens, human Tissue: lung Cell type: Epithelial
Thawing procedure:
The vial of cells should be thawed in a 37oC water bath with gentle agitation. For optimal performance, the vial should be thawed in under two minutes. Ensure that the cap of the vial did not loosen upon thawing, and re‐tighten as needed. Spray the vial with 70% EtOH and wipe off. Repeat. Using aseptic technique, add the contents of the vial to 9 ml of complete growth medium (without selection). Centrifuge for 5 min. at 125 x g. Aspirate the medium, being careful not to disturb the pellet. Resuspend in 10 mL of complete growth medium, and place into a culture vessel of your choice. Only add selection to the medium after 24 hours in culture.
Culture conditions:
Complete Growth Medium The base medium for this cell line is DMEM. For optimal growth and maintenance of selection, add the following components to the base medium: dialyzed fetal bovine serum to a final concentration of 10%. Selection Puromycin to a final concentration of 2 µg/mL
Culture temperature: 37 0 C with 5% CO2
Subculture:
Replace culture medium with selection‐free medium and incubate for up to 6 hours. Rinse the cells with PBS without cations, digest cells with 0.25% (w/v) Trypsin‐EDTA (0.53 mM)solution and split at 1:3 to 1:10 ratio.
Cryopreservation: Freeze slowly in complete growth medium supplemented with 5% (v/v) DMSO.
Mycoplasma: Negative
Fluorescence: Image of H1299/DSRED Labeled Actin Cell Line. Observing the cells climbing to the carry sheet glass, which are stained with DAPI.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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