人视网膜母细胞瘤y79 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：人视网膜母细胞瘤y79 cell line细胞株细胞系
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人视网膜母细胞瘤y79 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Catalogue No.: NTCC516725
Cell Line Name: Y79
Keywords: Human Caucasian retinoblastoma
Cell Line Description: Established from a primary tumour of the right eye of a 2.5 year old female Caucasian. The cells grow in clusters and exhibit reverse transcriptase activity.
Species: Human
Tissue: Retina
Growth Mode: Suspension
DNA Profile: STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 13,14
D5S818: 11,12
D7S820: 8,9
THO1: 6,9.3
TPOX: 8
vWA: 15,18
Subculture Routine: The percentage of viable cells is generally low upon resuscitation from frozen ampoules (often less than 50%). However the cells should recover in a few days. When starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube; slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 5 x 100,000 cells/ml in fresh medium. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Maintain cultures between 4-9x100,000 cells/ml; 5% CO2; 37°C.When in culture, stand flask up until cells show growth in large clumps. After approximately 3 days the flask can be laid down.
Requires 5% DMSO and 95% foetal bovine serum (FBS) as cryoprotectant.
Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype: 2n = 46, hypertriploid
Depositor: Dr S Clark, PHLS CAMR, Porton Down, Salisbury
Originator: No
Country: UK
References: J Nat Cancer Res 1974;53:347
Additional Bibliography: Not specified
Patents: None specified by Depositor
Research Council Deposit: No
Release Conditions: No
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
Catalogue No.: NTCC516725
Cell Line Name: Y79
Keywords: Human Caucasian retinoblastoma
Cell Line Description: Established from a primary tumour of the right eye of a 2.5 year old female Caucasian. The cells grow in clusters and exhibit reverse transcriptase activity.
Species: Human
Tissue: Retina
Growth Mode: Suspension
DNA Profile: STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 13,14
D5S818: 11,12
D7S820: 8,9
THO1: 6,9.3
TPOX: 8
vWA: 15,18
Subculture Routine: The percentage of viable cells is generally low upon resuscitation from frozen ampoules (often less than 50%). However the cells should recover in a few days. When starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube; slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 5 x 100,000 cells/ml in fresh medium. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Maintain cultures between 4-9x100,000 cells/ml; 5% CO2; 37°C.When in culture, stand flask up until cells show growth in large clumps. After approximately 3 days the flask can be laid down.
Requires 5% DMSO and 95% foetal bovine serum (FBS) as cryoprotectant.
Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype: 2n = 46, hypertriploid
Depositor: Dr S Clark, PHLS CAMR, Porton Down, Salisbury
Originator: No
Country: UK
References: J Nat Cancer Res 1974;53:347
Additional Bibliography: Not specified
Patents: None specified by Depositor
Research Council Deposit: No
Release Conditions: No
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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