JIMT-1 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

JIMT-1 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
JIMT-1 Cells

Cell line:
JIMT-1
NTCC no.:
NTCC-AC 589
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established from the pleural effusion of a 62-year-old woman with ductal breast cancer (grade 3 invasive, T2N1M0) after postoperative radiation in 2003; cell line was described to carry an amplified HER-2 oncogene and to be insensitive to HER-2-inhibiting drugs, e.g. trastuzumab (Herceptin)
Reference(s):
15007
Biosafety level:
1
Permissions and restrictions:
A

NTCC Cell Culture Data:
Morphology:
epithelial-like cells growing in monolayers; image; image
Medium:
90% Dulbecco's MEM + 10% h.i. FBS
Subculture:
split confluent culture 1:2 to 1:8 every 2-4 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 8-10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human flat-moded highly rearranged hyperdiploid karyotype with 6% polyploidy - 46-56<2n>XX, +1, +2, +2, +3, +5, +6, +7, +12, -13, -14, -15, -15, -16, -18, +17, +5mar, add(X)(p22.1), add(1)(q11), del(1)(q32.2), der(1)add(1)(p21)add(1)(q22), der(1)t(1;6)(p11;q1?1)t(6;5)(q25;?p13), add(2)(q11)x2, t(2;3)(q10;p10)add(2)(q25-31), add(3)(p11), der(4)t(?3;4)(?q24;p11), add(5)(q15), der(5)add(5)(p15.3)del(5)(q22), der(5)t(5;8)(p11;p11), add(6)(p11)add(6)(q23), der(7)t(1;7)(p13;q21), der(7)t(7;?12)(p11;p11), add(8)(p11), i(8q) add(9)(p11), der(9)add(9)(p11)add(9)(q23), add(11)(p13), der(11)dup(11)(p11p15)add(11)(p15), der(11)t(1;11)(q24;p12), add(12)(q21), der(12;?15)(p11;q11), add(17)(p11), add(17)(p13), der(17)add(17)(p11)add(17)(q21), inc; unresolved HSR markers present
Viruses:
PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, MLV -, SMRV -

【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net