HPD-1NR cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

HPD-1NR cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:
HPD-1NR
NTCC no.:
910-314
Species:
Syrian hamster (golden hamster) (Mesocricetus auratus)
Cell type:
pancreas ductal adenocarcinoma
Origin:
established from the pancreatic ductal adenocarcinoma of a female Syrian (Golden) hamster (Mesocricetus auratus) induced by treatment with BHP (chemical carcinogen); sister cell line of HPD-2NR (NTCC ACC 293); described in the literature to be homotransplantable (i.e. in other syngeneic hamsters) and to carry a mutation in exon 1 of the c-Ki-ras oncogene
Biosafety level:
1
Permissions and restrictions:
A

NTCC Cell Culture Data:
Morphology:
adherent epithelial cells growing mostly in monolayers (about 2-5% very large cells), but also with formation of a three-dimensional grid-like structure with some round, only loosly bound cells
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
split confluent culture before reaching confluence 1:2 to 1:4 every 3-6 days using trypsin/EDTA; seed out at about 2 x 106 cells/80 cm2; cells have a high metabolic activity acidifying the culture medium; exchange spent medium every 2 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-70 hours
Harvest:
cell harvest of about 6-7 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Fingerprint:
identical DNA profile with HPD-1NR multilocus probe (gtg)5
Species:
originally confirmed as Syrian (Golden) Hamster by species PCR and recently as Mesocricetus auratus origin by COI DNA barcoding
Cytogenetics:
near triploid highly rearranged karyotype with 36% polyploidy - 60-66<3n>XX, -X - hamster markers present - resembles published karyotype
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -


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