NIH-3T3 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

NIH-3T3 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:
NIH-3T3
Cat no.:
ACC 59
Species:
murine (mouse, Swiss mouse) (Mus musculus)
Cell type:
embryo fibroblasts
Origin:
contact-inhibited NIH Swiss mouse embryo; used in DNA transfection assays
Biosafety level:
1
Permissions and restrictions:
A

Cell Culture Data:
Morphology:
fibroblastoid, adherent cells growing as monolayer;
image

Medium:
90% Dulbecco's MEM (4.5 g/L glucose) + 10% h.i. FBS (or NCS)
Subculture:
seed out at 1-2 x 106 cells/175 cm2 flask; do not allow culture to become completely confluent; split culture at 1:10 every 4 to 6 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 20 hours
Harvest:
cell harvest of about 20 x 106 cells/175 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species:
originally confirmed as mouse with IEF of AST, G6PD, MDH, NP, by species PCR and recently as mus musculus by COI DNA barcoding
Cytogenetics:
murine hypertriploid karyotype with 3% polyploidy - 68(58-73)<3n>
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -


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