HIEC-6 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

HIEC-6 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Organism Homo sapiens, human
Tissue small intestine
Cell Type epithelium
Product Format frozen 1.0 mL
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1.
Disease normal
Age 17 to 19 week gestation
Applications Investigation of the regulation of normal intestinal cell proliferation, survival, stemness, as well as studies of the molecular mechanisms leading to lineage-specific differentiation. Also used for the study of the effects of hormones, growth factors, extracellular matrix molecules, drug metabolism and carcinogenic factors on small intestinal functions.
Frequently used as normal control cells for studies dealing with colorectal cancer cell lines.
Derivation HIEC-6 cells were isolated by thermolysin treatment of a human fetal small intestine.
Antigen Expression cytokeratin-8 +, cytokeratin-18 +, cytokeratin-21 +, mouse intestinal mucosa (MIM-1/39) +, aminopeptidase N (APN) +, dipeptidyl-peptidase IV (DPPIV) +
Tumorigenic no
Comments This is a continuously growing human intestinal epithelial cell line which expresses epithelial cytokeratins and functional markers for intestinal crypt cells. They exhibit typical intestinal crypt cell proliferative and undifferentiated characteristics.
Complete Growth Medium The base medium for this cell line is OptiMEM 1 Reduced Serum Medium (Gibco Catalog No. 31985). To make the complete growth medium, add the following components to the base medium:
• 20 mM HEPES
• 10 mM GlutaMAX (Gibco Catalog No. 35050)
• 10 ng/mL Epidermal Growth Factor (EGF)
• fetal bovine serum (FBS) to a final concentration of 4%
Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC® 30-2200) or 0.25% (w/v) Trypsin-0.53 mM EDTA solution (ATCC® 30-2101) to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation Freeze medium: Complete Growth Medium 95%; DMSO (ATCC 4-X), 5%. Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Volume 1.0 mL
STR Profile Amelogenin: X
CSF1PO: 11,12
D13S317: 11,14
D16S539: 12,13
D5S818: 11,13
D7S820: 8,11
THO1: 6,9
TPOX: 8,11
vWA: 14,15
Population Doubling Time 96 hours
Year of Origin 1995
References Perreault N, et al. Use of the dissociating enzyme thermolysin to generate viable human normal intestinal epithelial cell cultures. Exp Cell Res 224(2): 354-364, 1996. PubMed: 8612712

【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net