VRC8400 plasmid vector疫苗表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

VRC8400 plasmid vector疫苗表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
The VRC8400 expression plasmid was constructed as follows. The parental 1012 DNA vaccine plasmid contains the human cytomegalovirus (CMV) immediate early (IE) enhancer, promoter, and intron. To construct the CMV/R regulatory element, a SacII/HpaI fragment of the 1012 plasmid containing the majority of the CMV IE intron was replaced with a 227-bp EcoRV/HpaI fragment of the HTLV-1 R region. The resulting VRC8400 plasmid therefore contains the human CMV IE enhancer/promoter, followed by the HTLV-1 R region and a 123-bp fragment of CMV IE 3 intron. The splice donor in the R region and the splice acceptor in the CMV IE 3 intron serve as the pair of splicing signals. A codon-optimized version of the prM-E sequence from the DV 1 clone 45AZ5 was cloned by PCR and subsequently inserted into the VRC8400 plasmid.
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