hMLKL-Venus plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

hMLKL-Venus plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
expression in mammalian cells
Vector backbone pRetrX-TRE3G
(Search Vector Database)
Backbone manufacturer Clontech
Backbone size w/o insert (bp) 6600
Total vector size (bp) 8720
Vector type Mammalian Expression, Retroviral ; Tet-On retrovirus
Selectable markers Puromycin
GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) NEB Stable
Copy number Unknown
GENE/INSERT
Gene/Insert name human MLKL-Venus
Alt name full length human MLKL-Venus
Species H. sapiens (human)
Insert Size (bp) 1822
GenBank IDNM_152649.3
Entrez Gene MLKL (a.k.a. hMLKL)
Promoter Dox-inducible
Tag / Fusion Protein
Venus (C terminal on insert)
CLONING INFORMATION
Cloning method Restriction Enzyme
5′ cloning site ClaI (not destroyed)
3′ cloning site EcoRI (not destroyed)
5′ sequencing primer atcgatACCATGGAAAATTTGAAGC
3′ sequencing primer gaattcTTACTTGTACAGCTCGTCCATG
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net