74D‐694 strain酵母菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心

74D‐694 strain酵母菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心
The 74D‐694 strain is widely used to study and score for [PSI+]. Premature stop codons in genes of the adenine synthesis pathway, which are present in lab strain 74D-694, provide a convenient way to monitor switching of Sup35p to the prion state. In prion-free [psi−] cells, translation termination fidelity is high, leading to the production of truncated and nonfunctional Ade1p. As a consequence the [psi−] cells are unable to grow on adenine-free medium and accumulate a by-product of the adenine synthesis pathway that confers a red colony color when grown on other media such as YPD. Prion-containing [PSI+] cells, on the other hand, have a reduced translation termination activity that allows read-through of the ade1 nonsense allele and the production of functional full-length Ade1p protein, resulting in growth on adenine-deficient medium and the expression of a white colony color.
Genotype
MATa: ade1-14, trp1-289, his3Δ-200, ura3-52, leu2-3, 112, [psi−], [RNQ+]

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