Bacillus subtilis subsp. subtilis BioVector NTCC质粒载体菌种细胞基因保藏中心

Bacillus subtilis subsp. subtilis BioVector NTCC质粒载体菌种细胞基因保藏中心
Bacillus subtilis subsp. subtilis
Cat No.:NTCC692587

Designation: Marburg strain [ATCC 6051U,
CCM 2216, CCRC 10255, CCUG 163B, CFBP 4228, CIP 52.65,
DSM 10, IAM 12118, IFO 12210, IFO 13719, IFO 16412, IMET 10758, JCM 1465, LMG 7135, NCAIM B.01095,
NCCB 32009, NCCB 53016, NCCB 70064, NCFB 1769, NCIB 3610, NCTC 3610, NRRL B4219,
NRS 1315, NRS
744, VKM B501]
Deposited Name: Bacillus subtilis (Ehrenberg) Cohn
Product Description: Type strain. Bacteriophage host.
Medium
Nutrient agar or nutrient broth
Growth Conditions
Temperature: 30°C
Atmosphere: Aerobic
Propagation Procedure
1. Open the freezedried
vial according to enclosed instructions.
2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or
1.0 mL pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 slant, and/or plate.
5. Incubate all tubes and plates at 30°C for 24 hours.

This item has two colony types and both have been identified by 16S sequencing to be identical and
consistent with the genus and species.
Growth is observed after 24 hours. Growth may be poor in broth incubated statically, but heavy on agar.
This strain has been cited for use in bacterial resistance testing of latex paint (Machemer WE, 1979), blood
screening for phenylketonuria (Guthrie R, 1961), and accumulation of metal ions from aqueous solutions
(Brierley JA et al., 1990). This strain has also been cited as able to produce isoprene (Kuzma J et al., 1995).

【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net