Bifidobacterium animalis BioVector NTCC质粒载体菌种细胞基因保藏中心

Bifidobacterium animalis BioVector NTCC质粒载体菌种细胞基因保藏中心
Name:
Bifidobacterium animalis subsp. lactis (Meile et al. 1997) Masco et al. 2004
Synonym(s):
Bifidobacterium lactis Meile et al. 1997
DSM No.:
10140, Type strain
Strain designation:
UR1
Other collection no.
or WDCM no.:
CIP 105265, JCM 10602
Isolated from:
yoghurt
Country:
Switzerland
Date of sampling:
before 25.07.1995
Nagoya Protocol Restrictions:
History:
<- L. Meile <- U. Rüger, UR1
Genbank accession numbers:
16S rRNA gene: X89513
complete genome: CP001606
Cultivation conditions:
BIFIDOBACTERIUM MEDIUM , anaerobic, 37°C

BIFIDOBACTERIUM MEDIUM
Casein peptone, tryptic digest 10.00 g
Yeast extract 5.00 g
Meat extract 5.00 g
Bacto Soytone 5.00 g
Glucose 10.00 g
K2HPO4 2.00 g
MgSO4 x 7 H2O 0.20 g
MnSO4 x H20 0.05 g
Tween 80 1.00 ml
NaCl 5.00 g
Cysteine-HCl x H2O 0.50 g
Salt solution (see below) 40.00 ml
Resazurin (25 mg/100ml) 4.00 ml
Distilled water 950.00 ml
The cysteine are added after the medium has been boiled and cooled under CO2. Adjust
pH to 6.8 using 8 N NaOH. Distribute under N2 and autoclave.
Salt solution:
CaCl2 x 2 H2O 0.25 g
MgSO4 x 7 H2O 0.50 g
K2HPO4 1.00 g
KH2PO4 1.00 g
NaHCO3 10.00 g
NaCl 2.00 g
Distilled water 1000.00 ml
Summary and
additional information:
<- L. Meile <- U. Rüger, UR1. Yoghurt; Switzerland. Type strain.Taxonomy/description (6780, 6865, 8075, 9332). Sequence accession no. 16S rRNA gene: X89513, complete genome: CP001606. Murein: A11.21. (Medium 58, 37°C, anaerobic).

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