Aspergillus brasiliensis strain菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心

Aspergillus brasiliensis strain菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心
Strain Designation: WLRI 034(120) [CBS 733.88, DSM 1387, DSM 1988, IFO 9455, IMI 149007, NCPF 2275]
Deposited Name: Aspergillus niger van Tieghem
Product Description: An ampoule containing viable cells (may include spores and mycelia) suspended in
cryoprotectant.
The information recommended in this section is to assist users in obtaining living culture(s) for their studies.
The recommendation does not imply that the conditions or procedures provided below are optimum.
Experienced researchers may initiate the growth of a culture in their own way.
ATCC® Medium 336: Potato dextrose agar (PDA)
ATCC® Medium 325: Malt extract agar (Blakeslee's formula)
ATCC® Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions
Temperature: 20°C to 25°C
Atmosphere: Typical aerobic
Recommended Procedure
For freezedry
(lyophilized) ampoules:
1. Open an ampoule according to enclosed instructions.
2. From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL
with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g.,
overnight) rehydration might increase viability of some fungi.
5. Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended
solid or liquid medium. Include a control that receives no inoculum.
6. Incubate the inoculum at the propagation conditions recommended.
7. Inspect for growth of the inoculum/strain regularly. Viability is typically noticeable after 2 to 3 days of
incubation. However, the time necessary for significant growth will vary from strain to strain.
Colony and Cell Morphology: Colonies initially white or yellowish, mycelium growing rapidly producing a
dense layer of erect smoothstiped,
conidiophores terminated by globose vesicles bearing phialides
(uniseriate) or metulae with phialides (biseriate) which produce dry chains of conidia. Reverse pale to grayish
or greenish yellow. Vesicles radiate, initially pale, becoming dark brown to black. Conidia spherical, midtodark
brown, highly roughened with ridges and blunt or pointed protuberances, (3)
45(
6)
μm in diameter.
Sporulation may be inhibited when grown in vessels with reduced gas exchange. Colonies may exhibit
sectoring with areas of varying levels of sporulation. Use of freshly produced spores as inoculum should
reduce sectoring.
This strain was identified as belonging to the new species Aspergillus brasiliensis (see Varga et al. 2007
and Houseknecht et al., 2008.)
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net