S. aureus Xen36 strain菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心

S. aureus Xen36 strain菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心
Staphylococcus aureus Xen36
In vitro Characteristics
Genetic Characteristics
Staphylococcus aureus Xen36 was derived from the parental
strain Staphylococcus aureus ATCC 49525 (Wright), a clinical
isolate from bacteremia patient. S. aureus Xen 36 possesses a
stable copy of the modified Photorhabdus luminescens
luxABCDE operon at a single integration site on a native
plasmid. Xen36 should be stored at -80°C
Growth Characteristics
S. aureus Xen36 grows well in various media including Brain
Heart Infusion (BHI), Trypticase Soy Broth (TSB), and Luria
Bertani (LB) at 37ºC under ambient aeration. S. aureus Xen36
may also be grown selectively on medium containing 200
µg/ml kanamycin.
Colonial Morphology: On TSA agar plate, S. aureus Xen36
appears as small (~1.5mm), cream-colored, opaque, smooth,
circular colonies.
Growth Curve: Log-phase growth can be achieved after 1.5 to
3 hours of subculture in TSB broth at 37ºC with aeration at 200
rpm. An absorbance measurement at 600 nm (against a TSB
blank) of 0.5 is roughly equivalent to 1.0x108
cfu/ml of S.
aureus Xen36.
Virulence Factors
Hemolysis: β-hemolysis on TSA + 5% sheeps blood.
Capsule: Serotype 8.
DNAse: Positive.
NaCl: Tolerant via growth on Mannitol Salts Agar.
Coagulase: Positive in 4hrs.
mecA: negative.
0.1
1
10
105
106
107
108
-2 0 2 468
S. aureus - Xen36 Optical Density vs. Bioluminescence
OD600
A
bioluminescence
bsorbance at 600n
m
Photons/Sec
Time (hours)
0.1
1
10
107
108
109
1010
-2 0 2 4 6 8
S. aureus - Xen36 Optical Density vs. Viable Counts
OD600
A
CFU/ml
bsorbance at 600nm
CFU/ml
Time (hours)
Biochemical Profile
A biochemical profile was obtained for S. aureus–Xen36 using
the api 20 STAPH system available from bioMérieux.
Sugar Utilization Other Tests
D-Glucose + Nitrate Reduction +
D-Fructose + Alkaline Phosphatase +
D-Mannose + Voges Proskauer -
Maltose + α-methyl-D-glucoside -
Lactose + N-acetyl-glucosamine +
Trehalose + Arginine dihydrolase +
D-Mannitol + Urease +
Xylitol -
Raffinose -
Xylose -
D-Melibiose -
Sucrose +
Antibiotic Susceptibility
Disk Diffusion Data
Disk diffusion tests were performed according to methods
outlined in the NCCLS Approved Standard M2-A7.
Kirby-Bauer Disk Diffusion Test
Sensitive to: Resistant to:
Ciprofloxacin 5 µg Kanamycin 30 µg
Clindamycin 2 µg Penicillin 10 IU
Oxacillin 1 µg
Sulfamethoxazole 23.75µg /
Trimethoprim 1.25 µg
Tetracycline 30 µg
Chloramphenicol 30 µg
Vancomycin 30 µg
Gentamycin 10 µg
References:
1. Journal of Bacteriology, 2007, 8079-8087, Vol. 189, No. 22 2. J Immunol. 2007 Nov 1; 179 (9):6160-8
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