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Escherichia coli E. coli WS2572 (Xen14) BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥39865
  • 货  号:Escherichia coli E. coli WS2572 (Xen14)
  • 产  地:北京
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Escherichia coli E. coli WS2572 (Xen14) BioVector NTCC质粒载体菌种细胞基因保藏中心
E.coli Xen14 E.coli Xen14,1Vial. Storage:4℃
Biovector692558

Genetic Characteristics
Escherichia coli–Xen14 was derived from the parental strain Escherichia coli WS2572, a clinical isolate from Weihenstephan Culture Collection. E. coli-Xen14 possesses a stable copy of the Photorhabdus luminescens lux operon on the bacterial chromosome. Xen14 should be stored at -80°C
Growth Characteristics
E. coli-Xen14 grows well in Luria Bertani (LB) medium at 37ºC under ambient aeration. E. coliXen14 may also be grown selectively on LB agar containing 30µg/mL Kanamycin.
Colonial Morphology
On LB plates, E. coli-Xen14 appears as medium (2mm), cream, circular, colonies after 24 hours incubation at 37°C.
Growth Curve
Log-phase growth can be achieved after 1 to 1.5 hours of subculture in LB broth at 37ºC, shaking at 150-200 rpm. For the above broth culture conditions, an absorbance measurement at 600nm (against a LB blank) of 1.0 is roughly equivalent to 1.1x109 cfu/mL of Escherichia coli–Xen14 and the relative light intensity is 1.01photons/sec/cell.

Biochemical Profile
A biochemical profile was obtained for Escherichia coli–Xen14 using the api 20E system available from bioMérieux.

Antibiotic Susceptibility
Disk Diffusion Data Disk diffusion tests were performed according to methods outlined in the NCCLS Approved Standard M2-A7.
References
1. Infection & Immunity, Aug. 2005, 4512-4521
2. Antimicrobial Agents & Chemotherapy, Jan. 2001, p129-137


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