Mycoplasma pneumoniae FH strain肺炎支原体 BioVector NTCC质粒载体菌种细胞基因保藏中心

Mycoplasma pneumoniae FH strain肺炎支原体 BioVector NTCC质粒载体菌种细胞基因保藏中心
Mycoplasma pneumoniae FH
Cat No.: BioVector6923978

Name:
Mycoplasma pneumoniae Somerson et al. 1963
BioVector No.:
6923978
Strain designation:
Eaton Agent, FH
Isolated from:
monkey kidney tissue-culture fluids of the FH strain (Eaton Agent Virus), recovered in embryonated eggs from a student with atypical pneumonia.
Date of sampling:
before 07.09.2010
Genbank accession numbers:
complete genome: CP002077
Cultivation conditions:
SP4-Z MEDIUM + glucose, aerobic + 5% CO2, 37°C
Incubation time: 2-3 days

SP4-Z MEDIUM
The difference between this medium and "normal" SP4 medium is that the
yeast extract was left out and DNA was added instead. Since most mycoplasmas use either
glucose or arginine as their energy source one is added to the media. Do not combine both
in one medium, arginine can inhibit the growth of some glucose utilizers (unknown
reason).
For 200 ml of medium
PPLO broth 0.70 g
Tryptone 2.00 g
Bacto-Peptone (BD) 1.00 g
distilled water 130.00 ml
DNA (fish sperm; SERVA) 0.04 g
Adjust pH to 7.4; autoclave at 121C for 15 minutes.
Add aseptically:
CMRL-1066 medium (10x concentrated; GIBCO) 10 ml
Yeastolate (BD; 2%, autoclaved) 20 ml
Fetal bovine serum (heat-inactivated) 17 ml
Swine serum (heat-inactivated) 17 ml
As energy source add either
Glucose (50% aqueous solution) 2 ml
or
Arginine HCl (50% aqueous solution) 2 ml
For Ureaplasma add instead of Glucose or Arginine
Urea 0.4 g
For agar plates use only 126 ml of distilled water and add 2 g of agar. Medium containing
agar should be placed at 55oC before adding supplements.
Risk group:
2

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