pTT5-hSDC1-N20质粒载体BioVector NTCC保藏中心

Vector backbone：pTT5

• Backbone manufacturer
  NRC Biotechnology Research Institute

• Backbone size w/o insert (bp)4401

• Total vector size (bp)5300

• Vector type
  Mammalian Expression

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

GENE/INSERT

• Gene/Insert name
  SDC1
• Alt name
  Syndecan-1 N20 or 'del 1- 20' truncation mutant
• Species
  H. sapiens (human)
• Insert Size (bp)
  873
• Mutation
  Deletion of SDC1 aa2-21 (according to numbering in Fig. 2A of associated publication) and replaced with GS; R117Q (R95Q numbering as in Fig. 2A)
• Entrez Gene
  SDC1(a.k.a. CD138, SDC, SYND1, syndecan)

• PromoterCMV

CLONING INFORMATION

• Cloning methodRestriction Enzyme

• 5′ cloning siteunknown (unknown if destroyed)

• 3′ cloning siteunknown (unknown if destroyed)

• 5′ sequencing primerCCATACACTTGAGTGACAATGAC

• 3′ sequencing primerTATGTCCTTCCGAGTGAGAG