pAC-BETAipi大肠杆菌合成beta胡萝卜素质粒载体BioVector NTCC保藏中心

BioVector^TM pAC-BETAipi Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces -carotene in Escherichia coli

For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.

• Bacterial Resistance(s)
  Chloramphenicol, 25 μg/mL
• Growth Temperature
  30°C
• Growth Strain(s)
  Top10
• Growth instructions
  Grow liquid cultures on a platform shaker at 28 degrees Celsius for 2-3 days in darkness for best beta-carotene production, or grow on agar plates at room temperature for 3-7 days.
• Copy number
  Low Copy
• Gene/Insert name
  idi
• Alt name
  isopentenyl diphosphate isomerase
• Alt name
  ORF6
• Species
  Erwinia herbicola Eho10
• GenBank ID
  M87280.1

• Promoterendogenous promoter

• Cloning methodRestriction Enzyme

• 5′ cloning siteHindIII (not destroyed)

• 3′ cloning siteSalI (not destroyed)

• • Vector type
    low copy number bacterial cloning vector

  
  

BioVector^TMpAC-BETAipi, Plasmid BioVector53277. Gene/Insert:idi (Other)