pAC-BETA plasmid大肠杆菌胡萝卜素合成途径质粒载体BioVector NTCC保藏中心

Name名称：BioVector^TM PAC-BETA 大肠杆菌胡萝卜素合成途径质粒

Description

Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli.

For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.

Vector backbone BioVector^TMpAC-EHER

Backbone size w/o insert (bp)13581

Total vector size (bp)10609

Modifications to backbone

Deletion of adjacent 0.8- and 1.1 kB BamHI-BamHI fragments, and of a 1.1 kB Sall-Sal1 fragment served to remove most of the coding regions for the crtZ, crtX, and idi genes in plasmid pAC-EHER. The resulting plasmid, pAC-BETA, retains functional genes for geranylgeranyl diphosphate synthase (crtE), phytoene synthase (crtB), phytoene desaturase (crtI), and lycopene cyclase (crtY).

Vector typelow copy number bacterial cloning vector

Bacterial Resistance(s)Chloramphenicol, 25 μg/mL

Growth Temperature30°C

Growth Strain(s)Top10

Growth instructionsGrow liquid cultures on a platform shaker at 28 degrees Celsius for 2-3 days in darkness for best beta-carotene production, or grow on agar plates at room temperature for 3-7 days.

Copy numberLow Copy

Gene/Insert name crtE, crtY, crtI, crtB

Species Erwinia herbicola Eho10

GenBank ID M87280.1

Promoter endogenous promoters

Bacterial resistance原核抗性：
Selective Marker真核筛选标记：
Host Bacteria宿主菌：
Map图谱：
Sequence序列：
Culture condition培养条件：
Medium培养基：

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

http://www.biovector.net

pAC-BETA, Plasmid 53272. Gene/Insert:crtE, crtY, crtI, crtB (Other)