Desulfovibrio bizertensis BioVector NTCC质粒载体菌种细胞基因保藏中心

Desulfovibrio bizertensis BioVector NTCC质粒载体菌种细胞基因保藏中心
Name:
Desulfovibrio bizertensis
NTCC No.:
NTCC018034
Strain designation:
MB3
Isolated from:
marine sediment
Country:
Tunisia
near Bizerte
Date of sampling:
2003
Genbank accession numbers:
16S rRNA gene: DQ422859
Cultivation conditions:
HALODESULFOVIBRIO (POSTGATE) MEDIUM , anaerobic, 40°C

HALODESULFOVIBRIO (POSTGATE) MEDIUM
Solution A:
NaCl 25.0 g
K2HPO4 0.5 g
NH4Cl 1.0 g
Na2SO4 1.0 g
CaCl2 x 2 H2O 0.1 g
MgSO4 x 7 H2O 2.0 g
Na-DL-lactate 2.0 g
Yeast extract 1.0 g
Na-resazurin solution (0.1% w/v) 0.5 ml
Distilled water 980.0 ml
Solution B:
FeSO4 x 7 H2O 0.5 g
Distilled water 10.0 ml
Solution C:
Na-thioglycolate 0.1 g
Ascorbic acid 0.1 g
Distilled water 10.0 ml
Bring solution A to the boil, then cool to room temperature while sparging with 100% N2
gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas
atmosphere into anoxic Hungate-type tubes. During distribution continuously swirl the
medium to keep the grey precipitate suspended. Autoclave 15 min at 121C. Adjust pH
of the complete medium to 6.8 - 7.0, if necessary.
Risk group:
1

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