pIVEX2.4c原核表达载体质粒His标签 BioVector NTCC质粒载体菌种细胞基因保藏中心

pIVEX2.4c原核表达载体质粒His标签 BioVector NTCC质粒载体菌种细胞基因保藏中心
pIVEX His-tag Vectors are designed for high-level expression of Histagged proteins in the cell free RTS E.coli system. The vectors contain all regulatory elements necessary for in vitro expression with T7 RNA polymerase in the prokaryotic system. The introduction of either a N-, or a C-terminal His-tag provides a rapid method to purify proteins of interest. Cloning into pIVEX His-tag Vectors allows optimal protein expression in all RTS E.coli systems.
• pIVEX is the abbreviation for In Vitro EXpression. • The first number indicates the basic vector family • The second number indicates the kind and position of the tag – Even numbers mean tags, fused to the N-terminus – Odd numbers mean tags, fused to the C-terminus • Letters a, b, c indicate different reading frames.

Each pIVEX vector contains a hexa-His fusion tag to allow easy detection and purification of the expressed protein.
Use pIVEX 2.3 or pIVEX 2.3-MCS for fusing the gene with a C-terminal His-tag.
Use pIVEX 2.4(a-c) for fusing the gene with a Nterminal His-tag. For expression without tag use pIVEX 2.3 or pIVEX 2.3- MCS and incorporate the stop codon (TAA) at the end of the gene

【Supplier来源】BioVector NTCC Inc.
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【Website网址】 http://www.biovector.net